12/3/97

Dear All,

Does anyone have a better idea to detect intracellular calcium

than by using the potassium pyroantimonate method as suggested

in Hayat? We've tried it but we're not sure if it's precipitating

calcium or the cesium that we use to induce metamorphosis in our

experimental animals. Our thesis depends on it. Help!

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Name: Winston W Wiggins, Supervisor Vox:704/355-1267

CRC-Electron Microscopy Lab Fax:704/355-7648

Carolinas Medical Center Lab:704/355-7220

P.O. Box 32861

Charlotte, NC 28232-2861 USA Date: 12/3/97

E-mail: wwiggins@carolinas.org Time: 11:59:18 AM

Any idea is better than potassium pyroantimonate. Find the nearest

friendly electron probe analyst (Peter Ingram or Ann LaFurgey?) and see

whether they can help you, but it depends on Ca concentration that you seek.

Good luck!

aps2n@elvis.med.virginia.edu

Dear Winston,

I recommend the following methods:

1. Bichromate - Probst W., Histochemistry 85, 231-239, 1986.

2. Fluoride I - Ponie and Epel, J Histochem and Cytochem,

vol 35,no 9, 939-956, 1987.

3. Fluoride II - Vohringer P., Microscopy Res and Technique,

vol 31, 317-325, 1995.

I have been using the bichromate method mostly and with great success.

Antimonate is very close in x-ray energy to calcium so it is difficult to

support the findings of precipitated calcium by using EDX. This is not a

problem with bichromate.

Good luck!

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Rune Sundset

Dept. of Medical Physiology, Inst. of Medical Biology,

University of Tromsoe, N-9037 Tromsoe

Phone : +47 77 67 54 42 or +47 77 64 46 96 Fax : +47 77 64 54 40

http://www-users.fm.uit.no/~knutst/medfys/medfys.htm

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