Clarifying Tissue

11/24/97

I am interested to hear from individuals that have used methyl salicylate (?

conc.) to clarify tissue in particular skin.

Ramin Rahbari

313 998-3383

rahbarr@aa.wl.com

I have used Me salicylate to clear whole (gutted, 100mm sculpins) fish.

This was a pretty simple procedure, just dehydrate the fish in an ethanol

series, then transfer to 100% Me salicylate through a 3:1, 1:1, 1:3 EtOH:Me

sal. series. If parts stay cloudy, it is probably because they weren't

completely dehydrated. I used 1 to 3 days in each step, tissue pieces would

take less. When finished, the fish almost looked like they were made of

glass.

Phil

Philip Oshel

PO Box 5037

Station A

Champaign, IL 61825-5037

(217) 355-1143

oshel@ux1.cso.uiuc.edu

or poshel@hotmail.com