6/30/96
quantitate the number of gold particles. Does anyone know of any program
that can take an electron micrograph (or a scanned TIFF image of a
micrograph) and count the number of gold particles? If there is such a
program, can it be used to count gold particles of two different sizes?
Thanks,
Sanford Simon
Sanford M. Simon
Laboratory of Cellular Biophysics
Box 304
Rockefeller University
1230 York Avenue
New York, N.Y. 10021
212-327-8130 (voice)
212-327-8022 (fax)
simon@rockvax.rockefeller.edu (e-mail)
will count the particles in a field of view as well give you their relative sizes (areas).
Documentation is excellent and also available. NIH Image is public domain and free.
You can download by FTP a copy from the following sites:
zippy.nimh.nih.gov (site maintained by NIH & best place to look for current info)
www.amc.anl.gov (site maintained by Nestor for Microscopy & Microanalysis
usually a version behind but accessible)
log into either specifying a user name of
anonymous
and a password of
youremailaddress@yourhostcomputerdomain
at either site you will have to search the directory structure to find the
right place but it will be obvious.
on Zippy look under /pub/nih-image
on Nestor look under
/ANLSoftwareLibrary/4-MacShareware/Imaging/NIH-Image & SpinOffs
The current version is 1.60.
Nestor
Your Friendly Neighborhood SysOp
zaluzec@Sparc5.Microscopy.Com
TEMs and has functions for evaluating images of immunogold labelled
sections. It is sold by Soft Imaging Software, Gmbh, Germany, att.
Vasant Desai, Tel +49 251 798 000, Fax +49 251 798 00 99.
SIS just announced a website at http://www.soft-imaging-web.de
Finn-Mogens Haug
University of Oslo, Inst.bas.med.sci.
Department of anatomy E-mail: f.m.haug@basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78