6/11/97
I am working with cells growing on round cover slides. We are
looking for
an apporbiate holder to handle them in larger amounts during
immunohistochemistry. Something like a smaller version of the
staining
jars which are used for normal microscopic slides. Can someone
help me?
Thanks
Reinhard
. . . . . . . . . . . . . . . . .
. .
Dr. Reinhard Windoffer Fon: (00)49 (0)6131/39 3720
Universitaet Mainz Fax: (00)49 (0)6131/39 4615
Anatomisches Institut e-mail:
windoff@mail.uni-mainz.de
Becherweg 13
D-55099 Mainz
Germany
I use a very nice ceramic coverslip holder purchased from Thomas
Scientific.
You can also purchase glass jars of the appropriate size from the
same
catalog. Note that this item may be available from other vendors
as well.
This holder carries a dozen coverslips and has a metal handle
that you can
remove. It's actually a miniature version of the regular slide
holders. I
used it with square coverslips but regular sized round covers
should hold
without problem.
Sorry, I don't have the current reference at hand.
Good luck,
Michel
****************************************************
Michel Deschuyteneer, Ph.D. deschuyt@sbbio.be
Scientist Electron Microscopy Laboratory
SmithKline Beecham Biologicals
Rue de l'Institut, 89 B1330 Rixensart, BELGIUM
Tel: +32-2-656 9290 Fax: +32-2-656 8164
I have made round coverslip holders from fairly thick walled
flexible
polyethylene and silicone tubing by cutting it in half lengthwise
and then
cutting slits perpendicular to the length. When the tubing is
flexed in
the correct direction the slits are open for inserting the cover
slips.
When the tubing is held straight (in a pyrex tube or syringe
barrel) the
slits pinch closed and hold the cover slips. Use a tubing
diameter near
that of the cover slips. A cautionary note, if you plan to
critical point
dry samples held this way, be advised to test the tubing material
in the
CPD unit first. Some tubing types will turn into foam during
CPD.
good luck
cheers
ed
Edward J. Basgall, PhD
The Pennsylvania State University
Surface Chemistry Group
ejb11@psu.edu
Materials Research Institute Building Ph:
814-865-0493
University Park, PA 16802-7003 FAX:
814-863-0618
24 well
tissue culture plates.. Else the 12 well plates for larger ones.
Neelima Shah.
In my previous response I forgot to mention that a company here
in the US,
Tousimis Research Corp. also sold, at one time, a metal holder
designed for
CPD of stacks of cover slips. I have used it with good results
for CPD.
They come in two sizes. 13mm and 25mm?
Ph: 800-638-9558 or 301-881-2450. (No affiliation, only a
satisfied customer)
cheers
ed
Edward J. Basgall, PhD
The Pennsylvania State University
Surface Chemistry Group
ejb11@psu.edu
Materials Research Institute Building Ph:
814-865-0493
University Park, PA 16802-7003 FAX:
814-863-0618