12/3/96
am using diffusible tracers and cannot decalcify the bone. Any
suggestions would be welcomed on sectioning angle, temperature, knives,
etc. The piece that I need to section is about 5-7 mm square and I need
to have sections of at least 20 um in thickness.
Thanks in advance
Michael J. Lyon
lyonm@vax.cs.hscsyr.edu
The main problem I had when cutting mouse noses (snouts?) was getting the
sections to adhere to the slides during processing. My best results came
after reading a paper in The Journal of Histochemistry and Cytochemistry.
The article is "An Improved Method for Preparing Cryostat Sections of
Undecalcified Bone for Multiple Uses." Vol.38, No. 3. pp. 443-448.
Good Luck,
Kathy
Kathy Walters
Research Assistant III
Center for Microscopy Research
University of Iowa
85 EMRB
Iowa City, Iowa 52242
email: kwalters@emiris.iaf.uiowa.edu
fax: (319)335-8049