7/1/96
if one wants to avoid using DAPI (because of autofluorescence), what other
alternatives, with as much similar properties to DAPI as possible, do
exist?
Stamatis Pagakis snp@physiol.ox.ac.uk
University Laboratory of Physiology stamatis.pagakis@physiol.ox.ac.uk
University of Oxford, Parks Rd. FAX: +44 (0)1865 272469
Oxford OX1 3PT, United Kingdom Tel: +44 (0)1865 272551
move to visible light as the exicitation source. See the Molecular Probes
catalogue for detailed info, but if you must keep UV excitation, try
Hoechst 33258 or 33342, or trimethylpsoralin.
Robert J. Palmer Jr
rjpalmer@UTKUX1.UTK.EDU
CEB/UT
You might try one of the Hoescht dyes - for example Hoescht-33258. It is
available from Molecular Probes (tel. +31-71-5233431, email
76225.2203@compuserve.com, http://www.probes.com).
Hope this helps,
Rick Powell
Nanoprobes Inc
nano@NS1.LIHTI.ORG