From: dlb@u.Arizona.EDU (David Bentley)
I haven't used the Duco cement in a long time. Since it is a
solvent based cement, it will have a long drying time and the danger of
rewetting dry biological samples. If memory serves, there was no great
problem with outgassing after the glue was dried thoughly. It was used for
a larger sample so the beam was not on the adhesive often, but I do remember
trials where some damage was evident.
There was a fantastic, comprehensive, review article by Judy Murphy that
covers most mounting techniques well;
Judith A. Murphy. Considerations, Materials, and Procedures
for Specimen Mounting Prior to Scanning Electron Microscopic Examination.
Scanning Electron Microscopy 1982 II. SEM Inc. Chicago. 1982. 657-696.
Generally, with biological samples and impervious samples, I shy
away from solvent based adhesives. The former rewet and suffer surface
tension artifact on redrying, and the latter trap liquid adhesive underneath
and outgas for a long time. Usually, like to stay with the coated adhesives
like the transfer tabs (Avery "spot o' glue"), metal tapes, and for tiny
samples Scotch 850 polyester tape, so the samples don't sink, or form ripples.
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-- [ From: Charles A. Garber, Ph. D. GVKM07A@prodigy.com
On Sept. 3, Doug Davis asked about "Duco" brand cement:
"Duco" cement will certainly "work", however, there are much better
alternatives, "better" being defined from several perspectives:
a) Double sided adhesive conductive carbon sheets and tape will do the
same job (provided the samples being mounted are not too large) but
have the advantage of no outgassing. They are also "relatively" stable
under the beam, and since the substrate is already conductive, to
whatever degree one might have to apply gold or carbon to impart
surface conductivity, in this case, one would have to apply much less.
Cure time is not applicable, and the sample is instantly ready to be
put into the vacuum of either a sputter or carbon coater or the SEM.
Remember, Duco cement is certainly not conductive. Also, if not
completely cured (who has the time for a complete "cure" in a busy SEM
lab?), solvent vapors off-gas.
b) One can use products called "Leit-C-Plast" or even "Tempfix" which
have the advantage that more massive samples can be held in place and
the adhesive itself is conductive. Again, cure time is not an issue.
The only apparent "disadvantage" is that these other alternatives all
cost more money than "Duco" cement. On a "per sample" basis, we are
still talking pennies, but they do cost more.