3/31/98

Need your advice again! Can Spurr resin be etched like some of

the Epon-type resins can for Immuno work? If so does anyone have a

recipe? I have a difficult specimen, candida, that is great in

Spurr, so-so in Epoxy, horrible in Lowicryl and this is getting

frustrating. I don't know if Unicryl or LRwhite would work.

The problem with the hydrophilic resins seems to be that the

resin swells slightly as the sections are cut and the sharp

distinction between the resin and the wall of the candida causes a

split from the resin. The candida either fold over or fall out,

leaving so few good ones that I can't proceed to immuno work and hope

for any results.

Thanks,

Linda M. Fox

lfox1@wpo.it.luc.edu

embed yeasts in anything you want. I don't know the exact recipe, but

check out Laura I. Davis in Medline lit search. We did the EM for her on

cells from which she had removed the CW, and the sections were

beautiful. It's the thick wall that causes penetration problems.

Good luck,

Sara

saram@acpub.duke.edu

We used Unicryl resin for many years on several yeasts with good

results. We do not remove the cellwall of the cells but "etch" it a

little by an incubation with 0.1% meta periodate for 5 minutes prior

to the dehydration.

See the paper of Evert van Tuinen Journal of Histochem. & Cytochem.

vol. 35/3, page 327-333 (1987).

Good Luck.

K.A.SJOLLEMA

K.A.Sjollema@biol.rug.nl