2/20/97
Has anyone used Green Fluorescent Protein? I would like to use it for determining cell proliferation (because of the experimental set up, it is difficult to measure cell population directly).
Also, does anyone know of any dyes that do not affect cell viability but also will be retained by the cell long term (~2-3 weeks) without being metabolized or compartmentalized?
Eric Johnston
ericdj@seas.upenn.edu
Molecular Probes has a great web site - http://www.probes.com that you may
find helpful or call them for technical help at 541-465-8353 if you don't
have access to the www.
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602
Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth@dogwood.botany.uga.edu
double label anything, since it has a broad spectra. PKH from sigma is
another choice for tagging, etc., but I think that goes only to 10 days.
Good luck.
Rachel Teitelbaum
teitelba@aecom.yu.edu