10/9/98
Fischer - Zeit. wiss Mikrosk vol 29, 1912 p65
borax 5 grams
dissolve in 240 ml water
add 25 ml glycerine
add 40 grams gelatine,
dissolve with heat and heat until solution slightly thickens
remains fluid at room temperature
Pantin says glycerine jelly has an RI of 1.47
Apathys Gum syrup has an RI of 1.52
Picked gum arabic 50 grams
cane sugar........50 grams
distilled water 50 ml
dissolve over a water bath
add 0.05 grams thymol
said to set quickly as hard as balsam
recipes from "the Microtomist's Vade-mecum" by Bolles Lee 7th edition
1913.....
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Mel Dickson,
Director.
Electron Microscope Unit,
University of New South Wales.
Sydney NSW 2052 Australia
Phone (+612) 9385-6383
Fax (+612) 9385-6400
Website <http://srv.emunit.unsw.edu.au>
recipe was published in
Ztschr. f. wiss. Mikr., XXIX, p. 65, 1912.
The main advantage of this solution is, that it is liquid at RT... It's
said that it hardens rapidly.
This is the recipe as quoted in Langeron, M.: "Precis de microscopie",
1934, p. 603.
Distilled water 240 g
borax (= Na2B4O7): 5g
glycerin: 25g
gelatin: 40g
Dissolve all at RT. After solution put "some time" in a paraffin incubator.
This solution needs to be neutral...
I don't know the Ri of this medium...
Have you considered von Apathy's medium for your purpose?
Hope this helps...
Yvan Lindekens, Belgium.
yvan.lindekens@skynet.be