2/9/98

I am starting a study in which the size and distribution (may be shape) of

fat globules in milk samples can be of relevance. I wonder if I can

visualize these fat globules by LM (and if so, how, directly after some

staining) or if other techniques (I might think of SEM with cryofracture)

are appropriate.



I would be very grateful to any advice I receive.



Yours,



Antonio Molina-Garcia,

Inst. del Frio, CSIC

Madrid, SPAIN

Dr. P.D. Sanz

Engineering Department

Instituto del Frío (C.S.I.C.)

Ciudad Universitaria

Calle Ramiro de Maeztu, s/n

28040-Madrid

E-Spain



e-mai: psanz@fresno.csic.es



Telf: +34 1 5445607

+34 1 5492300



Fax: +34 1 5493627

Antonio,



I recall there were some papers published by Marc Horisberger back in the

late 70's about milk fat globules viewed in SEM (he was then working for

Nestle - of course!). I believe one of them was in J. Histochem. Cytochem.

He also used gold particles to label the globules, BTW. Sorry no specifics.

Using this in a citation index might help you get started on the right thread.

I hope this helps.

Regards,

Michel

Michel Deschuyteneer, Ph.D. deschuyt@sbbio.be

Scientist Electron Microscopy Laboratory



SmithKline Beecham Biologicals

Rue de l'Institut, 89 B1330 Rixensart, BELGIUM

Tel: +32-2-656 9290 Fax: +32-2-656 8164

Antonio:

We make gold-conjugated lipids which might be useful for this - they work

for labeling liposomes (see Adler-Moore, J. 1994. AmBisome targeting to

fungal infections. Bone Marrow Transplantation, 14, S3-S7; you can also see

the 1996 MSA abstract about gold-labeled liposomes as a pdf file on the web

at



http://www.msa.microscopy.com/MM96MeetingInfo/MM96Abstracts.pdf/MM96-289-C.pdf



from the MSA web site) and can be silver-enhanced for more visibility in

the EM or LM. Our web site catalog has more information at



http://www.nanoprobes.com/GoldLip.html



Hope this is helpful (excuse the product plug!)



Rick Powell

Nanoprobes, Inc.

rpowell@ns1.lihti.org

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