• 6 g glycerol (analytical grade)
• 2.4 g Mowiol 4-88 (Calbiochem #475904)
• 6 ml ddH2O
  
• Add 12 ml 0.2 M Tris buffer pH 8.5 and mix for ca half a day on a shaker.
• Let the mixture sit for 2 hrs. ( the Mowiol will not dissolve completely).
• Incubate at 50 degree centigrade for 10 min.
• Centrifuge mixture at 5000g for 15 min.
• Aliquot and freeze supernatant at -20 C until use.

Note: When thawing, make sure that the Mowiol solution has room temperature before mounting specimen. Otherwise many small airbubbles will form under the coverslip later. For protection from fading of fluorescent labels we add 0.1 % DABCO (Diazobicyclo-octane), the stuff that is also in the Molecular Probes Slow-Fade mounting medium. It is VERY cheap if you buy it just like that (Aldrich # D2,780-2).

================================ Christian Broesamle broesam@hifo.unizh.ch
Brain Research Institute
University of Zurich
August-Forel-Strasse 1
CH-8029 Zurich/Switzerland
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From: Greg Erdos (gwe@biotech.ufl.edu)

We obtain our Mowiol from Polysciences, Warrington PA

We add 0.5% paraphenylenediame (PPD) to it before freezing. (Longin, et al., 1993. Comparison of anti-fade agents. J. Histochem. Cytochem. 41:1833-1840.)