11/17/97

I am planning to do immunogold on brassica pollen grains. I

am looking for an adhesive that remain tacky when dried and

would hold pollen through out the process of washing,

fixing, immuno-treatment, postfixing, dehydrating and critical

point drying.

Any suggestions will be appreciated.

Thanks.

Ann Fook Yang

EM Unit,

ECORC

Agriculture and Agri-Food Canada,

Central Experimental Farm,

Ottawa, Ontario,

Canada K1A 0C6

Tel: 1-613-759-1638

Fax: 1-613-759-1701

e-mail: yanga@em.agr.ca

There is a product called Mikrostik sold by Ted Pella, Inc. (800-243-7765

is the toll-free number for Canada). Other vendors might sell this, also.

This is designed for small particle adhesion on stubs and is not supposed

to bury the things, like some tapes and tabs do. I have used it

successfully a couple times for things like phytoliths. I have no idea if

it would survive the washing, fixation, etc. processes, but it might be

worth a try.

I have no financial interest, etc.

Randy Tindall

Electron Microscope Laboratory

Box 3EML

New Mexico State University

Las Cruces, NM 88003

rtindell@NMSU.Edu

the glue from about a 30cm length of Scotch tape in about 10ml of

chloroform. Apply this to a clean abd shiny stub allow to d5ry and

sprinkle on or place pollen grains on the surface. Dry6 overnight in a

35oC oven, loghtly coat 8-10nm Au/Pd and away you go.

Patrick Echlin

Cambridge UK

pe13@cus.cam.ac.uk