9/11/96
I will appreciate it very much if anyone of you could tell me whether there
are photo scanners on the market that can be used to scan EM negatives into
the computer? I was told by a person from an image processing company that it
is possible to do this by using a transparency device. But I need to know if
there are people who are actually using those products for this purpose. I am
also interested in knowing the softwares, and printers that use photographic
paper. I do know there have been similar products that can be used for light
microscopy, e.g.,for flurescence microscopy, where the resolution of the
image is not a big concern. I do not know whether they can be used for
electron microscopy.
Thanks.
Yuhui Xu,MD,PhD
DFCI Core EM Facility
Harvard Medical School
Yuhui=Xu%RES%DFCI@EYE.DFCI.HARVARD.EDU
Transparency Adapter. With a good digital printer the results are closing in on
photographic quality .
Kate Connolly
Katherine.S.Connolly@Dartmouth.EDU
The combinations of equipment used for digital processing of EM negatives
will vary greatly from lab to lab. We have obtained excellent results
with the following combination:
1. Scan the EM negatives with a Leafscan 45 scanner (Leaf Systems, Inc.,
250 Turnpike Rd, Southboro, MA 01772; (508) 460-8300 -- however I think
they were bought out by some parent company). This can give very high
resolution (with consequent large files), taken up directly in Adobe
Photoshop on a Power Mac 8500. We learned about the Leafscan 45 from
Martin Muller's lab in Zurich.
2. Work with the EM files on the Mac with Adobe Photoshop 3.0 (crop,
brightness, contrast, size, resolution, grouping, labeling, and so forth
to infinity).
3. When the micrograph is exactly as you want it, print it from the
Macintosh on a Kodak XLS 8600 PS Printer. The results are glossy prints
that can rival anything you could do in the darkroom. The printer can
print B&W or color with very good quality. The "printing paper", however,
is quite expensive.
A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
<akc@umich.edu>
the Fujix Pictrograph 3000 digital printer (~$25K). With a top notch electronic
image, you'dswear it was a photo.
Walt Bobrowski
Parke-Davis Pharmaceutical Research
bobroww@aa.wl.com
A 45 T for negatives up to 4x5 inches, 30 bit color or B&W
A Scanmaker III for refection scanning; 36 bit color or B&W
a 35 t for fast scanning of 35 mmm size transparencies
We scan in the images so they are at least one megabyte (say 1024x1024).
We process the digital images in Photoshop 3.0 on a 100 mHz Pentium with
28 Mb of RAM and a 1.6 Gb disk.
For advanced enhancement we use a PiXision workstation.
But most of our images are now recorded digitally from the start. We
archive them on CD ROM.
For page makeup we mostly use CorelDraw.
For output we use a 600 DPI HP Laserjet for draft quality and a Tektronix
440 dye sublimation printer for photorealistic output. It does excellent
monochrome and briliant color, Cost AU$3.00 a page for mon o and AU$5.00
for color.
The system has been very well received by our users. The final result is
very professional and takes much less skill than conventional photography.
Mel Dickson.
m.dickson@unsw.edu.au