10/24/96
50um free floating section? It is brain tissue that has been fixed,
cryosectioned and returned to buffer. All wizardry is welcome at
this point !! Has anyone used Peldri II ? Would this be a
possibility? Thanks
Linda Fox lfox1@wpo.it.luc.edu
CPD: cut the end off of a BEEM capsule, cut out the center of the
capsule's cap, cut off the cap of another BEEM capsule, cut out the center,
put plankton netting over the end of the capsule with the lid, put in
sections, close lid; dehydrate, standing open end up in a Wheaton 4 mL
vial, add EtOH to capsule, withdraw from vial outside of capsule; put on
cut-off lid with netting,
put in CPD & dry:
___/ \__
| /________________\ |
lid on 1st capsule ---> | | | | <-----lid, removed from 2nd
| | | |
| |________|_______| |
|__\ | /_|
\ | /<------plankton netting
|
BEEM
Peldri: fluorocarbon, not made anymore.
or: dehydrate as usual, and dry from HMDS (Hexamethyldisilizane).
Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel@ux1.cso.uiuc.edu
(217) 355-1143 home
it more convient than Peldri and it gives similar to better results. There
are discussions about both which came across the list and I have archived
them at the web site listed at the end of this message. Click on the "Tips &
Tricks " button and look in the SEM section. Let me know if you cannot
access it and I will get it to you some other way
Scott Whittaker
sdw@biotech.ufl.edu
Sorry Philip O. but Peldri II is available. (Ted Pella Inc & P&S)
Those compounds do nasty things to the Ozone layer but, I am afraid, my
motorcar alone could produce enough nitrous oxides to exceed the damage done
by all the Peldri in the world.
The technical issue is the fragillity and the preservation of the section
for this Peldri II would be may first choice.
Jim Darley
Probing & Structure
(Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/