HI everybody,
Best wishes for this new year, hope that you will receive nice pictures...
There is a guy here in Strasbourg, who wishes to have a look on his oligodentrocytes in culture by SEM. For these purpose he uses antibodies coupled to gold to label the cells. My question is, what is the minimal size of the gold particles to have a chance to see them by SEM and the best way to prepare the sample (metallisation or not, carbon spputering, tension etc...) for SEM. The SEM we have is a XL 20 from Philips. Any related litterature is welcome as we are novice in this field.
TIA
Marc Schmutz
email: schmutzm@lear.u-strasbg.fr

We have a technical paper available on colloidal gold immunolabelling and SEM at our Web site.
The URL is:
http://www.mwrn.com/ebs/ebs.htm
I hope you find it helpful.
Steven Slap, Vice-President
75767.640@CompuServe.COM

Probably the most comprehensive general introduction to immunogold labeling, since it covers most applications, is vol. 1 and 2 from the series "Colloidal Gold: Principles, Methods and Applications" edited by M. A. Hayat (Academic Press, San Diego, CA; 1989 (Vol. 1 and 2) and 1991 (Vol. 3).
Richard D. Powell

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