2/10/97
of silicone gel at either EM or Light levels? As far as I know, the
silicone does not accept stain but thought I would learn if anyone has had
any experience with such specimens.
Regards, Don Cox, Goldmark Biologicals
goldmrkr@fast.net
staining of silicone in tissue. I happened to come across the following
reference which may be of interest:
Raso D et al. 1994. Light microscopy techniques for the demonstration of
silicone gel. Arch. Pathol. Lab. Med. 118: 984-987.
There also was an accompanying editorial in the same issue by Roggli et
al.
Daniel Luchtel
dluchtel@u.washington.edu
Several folks have asked for the response to my query about staining
silicone residues in tissue. Thanks so much for all the help and please
excuse my tardiness in getting this out to the members.
Regards, Don Cox
- - - - - - -RESPONSES- - - - -
Someone, a week or so ago (I deleted the message), asked for info about
staining of silicone in tissue. I happened to come across the following
reference which may be of interest:
Raso D et al. 1994. Light microscopy techniques for the demonstration of
silicone gel. Arch. Pathol. Lab. Med. 118: 984-987.
There also was an accompanying editorial in the same issue by Roggli et
al. - Daniel Luchtel <dluchtel@u.washington.edu>
- - - - -
Reply to: RE>Staining Silicone-Containing Tiss
We have been staining breast tissues from silicone implants for the past 5
years. We receive frozen tissue and cut slides for an immunofluorescent panel.
We do IgA, IgM, IgG, C3 and Fibrinogen. The sections are cut at 4 microns and
there have not been any cutting problems.
Carol Ann Bobrowitz <Carol_Bobrowitz.PATHOLOGY@qmail.path.mcw.edu>
Medical College of Wisconsin
- - - - -
Dear Don and Histonetters: There is an excellent reference entitled "
Light Microscopy Techniques for the Demonstration of Silicone Gel" in the
October, 1994 Volume 118 Issue of The Archives of Pathology and Laboratory
Medicine. I had the opportunity to hear the author, Dr. Dominic Raso,
speak on this subject. Bottom line -- Non-koehler, phase contrast and
darkfield illumination greatly enhance detection of silicone gel. He also
mentions a negative staining technique which is a 1 : 1 mixture of
Aqua-mount and black stamp pad ink. Oil red O is not very consistent.
Electron probe microanalysis also confirms the presence of silicon. Also,
sections need to be cut at 10 micron. My own experience with silicone gel
has been that it is highly refractile, not polarizable and no special stain
was needed. If you have difficulties locating the reference, I will be
glad to send you a copy. Linda Jenkins <jlinda@ces.clemson.edu>
MUSC
- - - - -
I once had to look for Si in the breast tissue of a woman who thought
she had been "poisoned" by her implants. I didn't find anything in
the breast tissue itself.
Almost forgot..... I did this with EDAX in the SEM, looking at a
section cut onto a plastic (Thermanox) coverslip, dried and carbon
coated. My explanation would be a bit fuller but I'm in the middle of
a conference. As I remember, a few relevant papers came up when I did
a lit. search using keywords like breast, silicon, etc.
Get back to me if you want more, I'll be freer next week.
Stephen Edgar
Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
email address: s.edgar@auckland.ac.nz