7/30/97

Hello all,



Is anyone familiar with stains and protocols for tissues embedded in

Spurr's low viscosity resin? I have been using toluidine blue on one

microns and need to achieve a greater differentiation among collagen fibers

and keratocytes within the stroma of the human cornea.



Thanks for any suggestions,



Dan Caruso c/o Eugene Gordon

MEDJET@worldnet.att.net

Eugene,

I use Spurr's exclusively for E.M. The stain I use on 0.5 micron

sections is Paragon. It is a combination of Toluidine Blue and Basic

Fuchsin and is a really beautiful stain. The reference for this is Dr.

Frieda Carson's book "Histologic Techniques In Electron Microscopy",

published by the American Society of Medical Technology, 1979. Hope

this helps.

Sharron G. Chism HT (ASCP)

Electron Microscopy Lab

Harris Methodist Hospital

Fort Worth, Texas

SharronChism@hmhs.com

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