11/12/97

consolidants into egg tempera based paint layers. The consolidants would

likely include animal glue, cellulosic ethers, and acrylics.

She has considered tagging the consolidant with one or more dyes to allow

visual microscopic examination of depth penetration in samples cut in

cross-section. Another means of assessing penetration would be micro-FTIR

step-scan analysis of bulk cross-sections or thin-sections (1-5 microns).

Here's a question for the TEM folk on the list. Can anyone think of a

feasible way to dope the consolidants with a metal(s) to allow mapping of

depth penetration by SEM-EDS, TEM, or another technique?

I have only a limited knowledge of the use of metal-labelled antibodies to

mark specific antigens using EM, and know this application I describe is

quite different.

Thanks for your assistance with this inquiry.

James Martin

James.S.Martin@williams.edu

I have experience with penetration of glues, preservatives, etc. into wood,

and the best label was bromide. This shows up well in EDX, is completely

soluble and you can brominate most organic compounds quite well. We also

used it the trace the movement of epoxy resin in prepreg layup parts

(composite materials). Brominate the consolidant, then trace the presence of

bromine with an EDX linescan or careful P/B analysis of bromine. It works

equally well in TEM for individual wood cell wall layers (brominate the

lignin) or SEM for overall penetration depths.

Mary Mager

Electron Microscopist

Metals and Materials Engineering

University of British Columbia

6350 Stores Road

Vancouver, B.C. V6T 1Z4

CANADA

tel: 604-822-5648

fax: 604-822-3619

mager@unixg.ubc.ca

substrates, we can label some of the substrates with our 1.4 nm Nanogold

cluster label, which we have cross-linked to a number of small molecules

before (hope this doesn't sound too much like a commercial plug - we are

interested in this type of experiment from a research perspective as well).

Rick Powell

Nanoprobes, Incorporated

rpowell@ns1.lihti.org