9/30/97

I'm trying to find a protocol that describes the TOTO or PATOTO

(not potato!) technique for fixing plant specimens for SEM. I've tried

doing a literature search and all I get are references to Dorothy and Oz,

just kidding. I've read about the procedure but I cannot find a good

descriptive protocol.

Thanks for helping with this.

I'll get that pretty picture, and it's little dog, too.

Hoping a house doesn't fall on you,

Paula = )

Paula Sicurello

UC Berkeley

Electron Microscope Lab

psic@uclink4.berkeley.edu

"Thiocarbohydrazide-mediated osmium binding: a technique for

protecting soft biological specimens in the scanning electron

microscope" by Robert O Kelly, etal. in Principles and Techniques of

Scanning Electron Microscopy. vol 4. 1975. ed by M.A.Hayat.

ISBN 0-442-25686-8. This method enhances osmium binding and therefore

reduces charging and heating of difficult to gold -coat structures. I

am not sure how well this would work with plant material. Of course

you may be refering to another method.

Hank Adams

New Mexico State University

Las Cruces, NM

hadams@cnmailsvr.nmsu.edu

I published a paper on a modification of the TOTO technique which

works well on plants. The reference is: FOOD STRUCTURE, Vol.12 (1993)

pp.475-482. If you wish to talk to me about it, feel free to contact me

directly.

Bill

William R. McManus

Electron Microscopy Facility

Department of Biology

Utah State University

Logan UT 84322-5305

1-801-797-1920

billEMac@cc.usu.edu