Resource for Informational Updates
2012 » Jan | Feb | Mar | Apr | May | Jun | Jul | Aug | Sep | Oct | Nov | Dec
ICBR Seminar Series on Platforms for Mid-Level Gene Expression and Genotyping - Two Luminex Seminars
Date: Wednesday, May 16, 2012
Times & Location: 9:00-10:30 a.m. (in Room 451A/B CGRC) and
1:00-2:30 p.m. (in Room R2-265 ARB)
(Seminar will be presented twice.)
Have your multiplexing and high-throughput, too...
Interested in detecting 2 to 500 transcripts per sample and processing1,000s of samples per day?
Learn how! Join the seminar by Stephen Angeloni, PhD fromLuminex Life Sciences.
TheLuminexFlexScriptLDA assay is:
Sensitive: inputs as low as <1,000 cells or <100ng Total RNA
Reproducible: robust performance results
Accurate: high correlation to real-time PCR
Contact National Sales Manager, RafaelCasiano (rcasiano@luminexcorp.com or Cell: 813-507-0527) for more information or to RSVP for a seminar.
ICBR Seminar Series on Platforms for Mid-Level Gene Expression and Genotyping - Applied Biosystems
Date: Wednesday, May 9, 2012
Time: 12:00-1:00 p.m.
Location: Academic Research Bldg. (ARB), Room R2-265
Refreshments will be served*, no RSVP required
Exploring the Next Generation of Real Time PCR
Speaker: Jonathan Fisher, Technical Sales Specialist, Next GenQPCR Systems and Applications
What if………………………..
· You could profile ~750MicroRNA’s in 48 samples in one 8 hour day?
· You could perform High-Throughput Genotyping for less than 8 cents per Genotype?
· You could distinguish between 5 and 6 copies of a gene via Digital PCR?
· You could perform over 12,000 reactions in Real-timePCR in 2.5 hours or over 110,000 reactions in an 8 hour day?
· You could do ALL OF THIS ON 1 PLATFORM!!!
Learn about new technology that facilitates Real-time PCR-based applications on a state-of-the-artnanofluidic plate, running up to 3,072 reactions in parallel. Flexible formats scaled to research needs enable the rapid analysis of tens to hundreds of genes or gene products across hundreds to tens of thousands of samples, for common applications such as:
· Genotyping as part of a drug / therapeutic discovery pipeline.
·SNP QC and screening.
· DigitalPCR for rare allele detection.
·MicroRNA profiling.
· Biomarker confirmation and screening.
· Gene expression quantification.
·GWAS follow-up studies
·SNP sample tracking for biobanking.
·SNP fingerprinting for ancestry.
· Marker assisted selection (MAS).
· Quantitative trait locus (QTL) mapping.
· Pathogen Detection.
For more information please contact your Life Technologies Instrument Rep, Shelina Folsom 407.782.0693
ICBR Cellomics Seminar Series - ForteBio Seminar
Date: Wednesday, May 9, 2012
Time: 12:00-1:00 p.m.
Location: Cancer Genetics Research Building, ICBR room 184
Pushing the Boundaries of BLI: Emerging Applications for Label Free Assays on the Octet and BLItz Systems
Speaker: Michele Halvorson
Lunch will be served*, no RSVP required
Come Learn about the latest advances, applications, products and systems available from ForteBio.
Useful for interactions of DNA, RNA, Protein, Antibodies, Fragments, Small Molecules, Virus andVLP’s for both kinetic andquantitation analysis!
Specificity of binding
·Epitope binning / Antibody pairing
· Yes / No binding confirmation
Protein Concentration
·ELISA’s in 30 minutes
· Wide dynamic range / fewer dilutions
Affinity characterization
· Affinity and on / off rates
· Rank order
For more information please contact Dan McElroy dmcelroy@fortebio.com 678.428.2387
Cellomics Seminar Series - Protein Simple Lunch & Learn
Date: Tuesday, May 1, 2012
Time: 12:00-1:00 p.m.
Location: Cancer Genetics Research Building, ICBR room 184
Simon™ and the Simple Western™ and NanoPro™
Speaker: Deborah Pritchett, Ph.D.
Simon™ and the Simple Western™ Automated Western Blots
Come to the seminar and see how you can do chemi-westerns without running gels and blots!
NanoPro™
Are you studying protein post translational modifications and are sample limited? Come and see how you can obtain quantitativemeasurments on subtle protein modifications requiring only a few hundred cells.
*To reserve your box lunch email Rick Estes at: rick.estes@proteinsimple.com
Cellomics Seminar Series - Roche Seminar
Date: Wednesday, April 25, 2012
Time: 12:00-1:00 p.m.
Location: Cancer Genetics Research Building, ICBR room 184
Good Cell Culture Techniques
Speaker: Dr. Charles Hardwick, Roche
This seminar is an engaging presentation on best practices in cell culture, including monitoring of cell growth, avoidance of contamination, and options for automation.
Refreshments will be served.*See Disclaimer
Cellomics Seminar Series - PerkinElmer Inc. Seminars
Date: Wednesday, April 18, 2012
Time: 11:15 AM - Lunch Provided; RSVP Evelyn Griffin evelyn.griffin@perkinelmer.com (see disclaimer)
11:30 AM to 12:15 PM - Alpha technology: increasing your sensitivity, throughput and data quality.
Speaker: Jimmy Barbee, SE Application Scientist, PerkinElmer Inc.
Increase Sensitivity and Throughput-96 well and 384 well formats
- No wash replacement for conventional ELISA
- Easy method for detecting molecular interactions (DNA, RNA, protein, glycosylation, ubiquitination, and more)
- Validated kits for epigenetic targets
- Quantitative method for endogenous phosphorylation studies
12:15 PM to 1:00 PM - Tyrimide Signal Amplification Systems for IHC and ISH: See what you've been missing!
Speaker: Jimmy Barbee, SE Application Scientist, PerkinElmer Inc.
1:00 P M to 1:45 PM - Novel Fluorescence Agents optimized for translational in vivo imaging, providing greater biological, functional and physiological information.
Speaker: Marko Andric, Pre-Clinical Imaging Specialist, PerkinElmer, Inc.
Location: Cancer Genetics Research Building, ICBR room 184
Coffee with the speakers following the talks at 1:45 PM.
Cellomics Seminar Series - GE Healthcare Life Sciences Seminar
As part of ICBR’s Cellomics Series, please join us for a series of presentations by GE Application Specialists and local scientists describing their most recent studies. Label-free analysis using Biacore™ and MicroCal™ systems provides comprehensive characterization of biomolecular interactions.
Date: Wednesday, March 28, 2012
Time: 9:30 AM to 11:30 AM - Seminar
Location: Cancer Genetics Research Building, ICBR room 184
Label-free Analysis Seminar
Eric Roush, Ph.D. - Biacore Field Application Specialist, GE Healthcare Life Sciences
Stoyan Milev, Ph.D.– MicroCal Field Application Specialist, GE Healthcare Life Sciences
Virus Antibody Interactions:
Larry Tartaglia, Graduate Student
UF College of Medicine, Dept. of Biochemistry & Molecular Biology
Protein Dynamics & Ligand Interactions:
Dr. Antonette Bennett, Ph.D.
UF College of Medicine, Dept. of Biochemistry & Molecular Biology
Questions? Contact your account manager: Joanne.Lopez@ge.com
Date: Tuesday, March 27, 2012
Time: 12:00 PM to 1:00 PM - Seminar - Lunch served (see disclaimer)
1:00 PM to 1:30 PM - Q&A/Demo (H4 multi-mode reader, Take 3 low-volume plate, 405 Touch washer)
Location: Cancer Genetics Research Building, ICBR room 184
Proximity Assay Technologies: FRET, TR-FRET, and Alpha Assays
Dr. Paul Held can answer questions on microplate assays including:
- Gene Expression
- Ion Channel assays
- Cell migration assays
- Mag/Poly bead assays
- Reactive Oxygen Species
- Luciferase reporters
- UV/vis, fluor, lum assays
-FRET or TR-FRET
Free $50 iTunes Raffle. Register at event or email SandovalJ@BioTek.com to receive pre-registration raffle form.
ICBR Technology Spotlight: Data Analysis and Research Computing
ICBR Technology Spotlight
A New Constellation for UF Biocomputing:
Galaxy - an open platform for accessible computation
Date: Friday, March 23, 2012
Time: 1:00 PM to 4:30 PM
Location: Cancer and Genetics Research Complex Auditorium
1:00 – 1:15: Welcome and Introduction of Speakers (Bill Farmerie, Assoc. Director, ICBR)
1:15 – 1:30: Research computing at the University of Florida (Elias Eldayrie, VP and CIO, UF)
1:30 – 1:45: Research computing and the bio-cluster supporting the life sciences (Erik Deumens, Director, UF Research Computing)
1:45 – 2:45: Galaxy: Making “high-end” computational biology more accessible (James Taylor, Assistant Professor, Biology/Mathematics and Computer Science, Emory Univ.)
2:45 – 3:00 - Break
3:00 – 3:30: Galaxy: RNA-seq demonstration (James Taylor, Assistant Professor, Biology/Mathematics and Computer Science, Emory Univ.)
3:30 – 3:50: Local Galaxy implementation (Oleksandr Moskalenko, Biological Applications Specialist, UF Research Computing)
3:50 – 4:10: Galaxy: Metagenomics demonstration (Matthew Gitzendanner, Assoc. Scientist, Biology and UF Research Computing)
4:10 – 4:30: Questions and Closing Remarks (Bill Farmerie, Assoc. Director, ICBR)
Proteomics Seminar Series: Life Technologies Seminar
Speaker: HealthBalcer Ph.D., Product Manager, Life Technologies
Date: Thursday, March 8, 2012
Time: 1:00 PM
Location: Cancer Genetics Research Building, ICBR room 184
Select and assay the proteome using ProtoArray® and ProtoPlex™ Technologies
Cell signaling mechanisms that underpin diseases remain poorly understood because of the difficulty of studying the associated proteins. Large scale protein studies have been inhibited by the poor availability of protein content and reliable platforms to rapidly assay protein functions.
We will present how a large breadth of protein content, over 9,000 human proteins generated through our high throughput protein production system, have been readily tested in planar array and bead array based platforms.The ProtoArray® microarray technology relies on a printed planar array to provide broad based coverage of the proteome for a range of discovery applications (protein-protein interactions, enzyme substrate identification, autoantibody biomarker discovery etc). Our recently developed antigen-based ProtoPlex assay compliments the ProtoArray® microarray by allowing hundreds of antigens to be selected from our human proteome collection for testing in the same assays. This enables everyday scientists to test hundreds to thousands of proteins to discover novel functions and/or validate biological relevance.
In this seminar, we will present how the two technologies in combination with access to broad content have been used to study immune response signatures in autoimmune diseases and cancer.
*Refreshments served, no RSVP required
Proteomics Seminar Series: Thermo Fisher Scientific Seminar
Utilization of enrichment methods and quantitative proteomics
techniques to monitor cellular regulation
Speaker: Brian Reese, Ph.D., Proteomics Applications Specialist, Thermo Scientific, Pierce Protein Research
Exploring Quantitative Differences in Serum Stimulated
HeLa Cell Lysates Using Hybrid Orbitrap Systems
Speaker: Craig P. Dufresne, Ph.D., Senior Technical Instructor for Mass Spectrometry, Thermo Fisher Scientific, Unity Lab Services
Date: Wednesday, March 7, 2012
Time: 11:30 AM to 1:00 PM-- Both seminars will be presented with lunch served (see disclaimer)
Location: Cancer Genetics Research Building, ICBR room 184
*RSVP to: Gerald.koncar@thermofisher.com
Abstracts:
Utilization of enrichment methods and quantitative proteomics techniques to monitor cellular regulation
Mass spectrometry provides users with an unparalleled ability to look at cellular regulation in terms of monitoring both protein expression levels and post translational modification. However, current proteomics analysis is not without significant limitations caused by the dynamic range of proteins in a cell, the sensitivity and speed of instrumentation, and the need for contextual information for data analysis. By combining quantitative MS-analysis, using methods such as MRM/AQUA, SILAC, orTMT isobaric tags, with specific sample fractionation methods, the quality and depth for proteomic analysis can be greatly improved. Methods of sample fractionation described include enrichment of; phosphopeptides, kinases, sub-cellular organelles,cysteine containing peptides, and cell surface proteins. A novel approach for removing detergents from samples is also examined.
Exploring Quantitative Differences in Serum StimulatedHeLa CellLysates Using HybridOrbitrap Systems
HeLacells cultured for 24 hours in low serum (0.5%) conditions and then serum stimulated (10%) prior to harvesting will be used to illustrate quantitative differences compared to normal growth conditions. Various methods for acquiring label freequantitation data on hybridOrbitrap high resolution mass spectrometers will be used. Discoveryquantitation using recursive base peak framing in SIEVE and targeted quantitation-high resolution extracted ion chromatograms in Pinpoint will be used for data processing and comparative presentations of the data.
EQUIPMENT AND SOFTWARE SEMINARS
Agilent Technologies -- Genomics Workshop
Speaker: Victor Miller
Date: Tuesday, February 28, 2012
Time: 1:00 PM to 2:30 PM - AgilentSureSelect and HaloPlex Target Enrichment Overview and Update
2:30 PM to 4:00 PM - Agilent eArray Design for Custom Microarrays in DNA and RNA Applications
Location: Cancer Genetics Research Building, ICBR room 184
Refreshments* will be provided, please RSVP to victor_miller@agilent.com
Talk 1: AgilentSureSelect andHaloPlex Target Enrichment Overview and Update
Target Enrichment Applications utilizing next-generation sequencers are powerful genetic tools to enable rabid discovery of SNPs, InDels and fusion transcripts etc in a focused set of genes, exons and/or genomic regions, as well as are emerging molecular diagnostic tools to screen known mutations and identify novel mutations in genetic diseases including cancer. With over 150 publications citing SureSelect platform in just over 2 years, Agilent Technologies is the leading provider for target enrichment applications with the broadest product portfolio.
Talk 2: Agilente Array Design for Custom Microarrays in DNA and RNA Applications
Custom microarrays are ideal tools to facilitate rapid DNA and/or RNA analysis focusing on specific sets of genomic regions and/or transcripts/exons in research and diagnostics applications. With over a decade of expertise in high-performing microarray platform, Agilent Technologies relies on its free eArray design software to empower scientists with utmost flexibility and accuracy in designing custom microarray in any species.
Life Technologies/Applied Biosystems Presents: Expand the Boundaries of Your Research with the QuantStudio 12K Flex Real-time PCR System
Speaker: Jonathan Fisher, Technical Sales Specialist,NextGenQPCR Systems & Applications
Date: Wednesday, February 22, 2012
Time: 12:00 PM to 1:00 PM - Introducing the NEWQuantStudio12K Flex real-TimePCR System!
Location: Cancer Genetics Research Building, ICBR room 184
Life Technologies is takingqPCR to the next level:
Designed for maximum throughput, flexibility, and scalability, the QuantStudio12K Flex Real-Time PCR System sets the new standard for what a real-time PCR instrument should be. Combining flexible throughput capabilities with streamlined workflow, intuitive software, and a state-of-the-art industrial design, this is one instrument that can take you from the targeted discovery through confirmation and screening, and everything in between.
· Maximum throughput, minimal resources
TheQuantStudio12K Flex System can simultaneously run up to four 3,072 reactionQuantStudio12K FlexOpenArray Plates in about 4 hours. A streamlined workflow and automated platehandling minimize start-up and hands-on times.
· Outstanding flexibility
TaqManAssays are available for all five thermal cycling blocks: OpenArray,TaqMan Array Card, 384-well, and standard or FAST 96-well blocks. Block changes typically take less than I minute and require no tools, providing the ultimate in format flexibility. Achieve consistent, high-quality results across differentplating formats as your research progresses.
· Scales to your research
With five interchangeable thermal cycling blocks, you can analyze from 1 to over 12,000 data points per run in the assay format that’s right for your project-today and in the future.
For more information contact: Jonathan.Fisher@lifetech.com
Clontech and Illumina Joint Seminar-- Presenting information about the new SMARTer™ Ultra Low RNA Kit for Illumina™ Sequencing and iDimerize™ Systems
Please come by and listen if you are interested in makingcDNA libraries for next-generation sequencing onIllumina instruments, or inducing proteinoligomerization and cellular localization.
Date: Tuesday, February 21, 2012
Time: 10:00 AM to 12:00 PM-Enabling single celltranscriptome analysis using SMARTer technology.
· Start with just 100 pg of RNA or a single cell.
· Generate high yields of full-length ds cDNA forIllumina paired-end library sample prep.
12:00 PM to 1:00 PM - *Lunch, please RSVP to jonathan_doose@clontech.com
1:00 PM to 2:00 PM –Controlling protein-protein interactions with the iDimerize system.
· Induce protein oligomerization and cellular localization.
· Specific induction occurs within seconds of adding a small molecule ligand.
Location: Cancer Genetics Research Building, ICBR room 184
For more information contact JonathanDoose 800.662.2566, ext. 7812 jonathan_doose@clontech.com
Illumina is a registered trademark of Illumina, Inc.
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Your use of these products and technologies is subject to compliance with any applicable licensing requirement.
Bio-Rad Laboratories Seminar--Droplet Digital PCR
Speaker:Yihua Che, Ph.D., Bio-Rad Laboratories
Date: Wednesday, February 8, 2012
Time: 10:00 AM to 11:00 AM - Seminar
11:00 AM to 11:30 AM - Q and A
Location: Cancer Genetics Research Building, ICBR room 184
Abstract:
Precision has been a rising challenge for current PCR/Real-TimePCR user as the research expanded into an area where rare variation needs to be addressed. Researchers conducting disease linkage study were struggled to achieve a reliable precision level as well as throughput with current technologies. What difference will it make if you could identify copy number variation (CNV), mutations at a single molecule level, or as low as 10% mRNA difference? What technology can provide that kind of precision and accuracy?
PCRtechnology has revolutionized into its third generation, the Digital PCR. Droplet DigitalPCR technology, with more evenly distributed molecule pairs, can provide absolute measure of target molecules with unrivaled quantitative resolution and sensitivity. Droplet DigitalPCR usesmicrofluidics to partition each sample into 20,000 to millions of one-nanoliter droplets, each of which contains a separate PCR. The droplets are streamed in single file past a detector to count the negative and positive reactions. No standard needed. The principles and instrumentation will be addressed in details as well as applications including CNV, mutation detection and gene expression in this seminar.
FLOWJO--Flow Cytometry Data Analysis Seminar
Speaker: IsaiahHankel Ph.D., Application Scientist
Date: Monday, February 6, 2012
Time: 10:00 AM to 11:00 AM - Basic Functionality, Groups, Layouts, Tables, Batching
11:00 AM to 12:00 PM - Compensation, Transforms, Advanced Analysis Platforms
Location: Cancer Genetics Research Building, ICBR room 184
For more information about the seminar contact Neil Benson nbenson@ufl.edu
Molecular Devices Seminar and Demonstration- The Increasing Variety and Role of Microarrays in Science Today
Speaker: Sean Carriedo, Ph.D., Field Applications, Molecular Devices
Date: Wednesday, February 1, 2012
Time: 10:00 AM to 11:00 AM - Seminar with Q&A
11:00 AM to 11:30 AM - Introduction to the hardware and “how-to-scan-a slide” tutorial
11:30 AM to 1:00 PM - Lunch*, seating is limited, please RSVP todan.amend@moldev.com
Location: Cancer Genetics Research Building, ICBR room 184
Abstract:
A microarray is an array of a large number of samples laid out in an orderly arrangement onto a standard laboratory slide. Microarrays can range from a few hundred spots on a slide to as many as a few million samples on the slide. The development of microarrays in the mid 1990’s stems from the need to find a more high throughput investigative methodology than the grind-and-bind techniques of Northern, Southern, and Western blots that were widely in use at the time. By the late 1990’s, the mainstream introduction of DNA-based gene expression arrays had become extremely popular. Over the past 10 years, microarrays have evolved from its gene expression analysis roots into a variety of different types of microarrays including Comparative Genomic Hybridization arrays, Protein arrays, Tiling arrays, Tissue arrays,microRNA arrays, and Chemical arrays among others. Today, microarrays are an important investigative tool in a variety of areas including Cytogenetics, Oncology, Proteomics, Toxicology, Fish Farming, and Diagnostics.
Today’s talk will focus on an introduction to microarrays and the processes involved in both scanning and analyzing microarray slides using the GenePix scanner and software ecosystem from Molecular Devices.
For more information contact: Sean Carriedo, Ph.D., BioReseach Applications Scientist sean.carriedo@moldev.com
Proteomics Seminar Series: AB SCIEX - Quantitative Biology Seminar
Join us for our workshop focusing on advanced quantitative proteomics workflows on modern accurate mass technology platforms.
Date: Thursday, January 26, 2012
Time: 12:00 Noon to2:30 PM
Location: Cancer Genetics Research Building,ICBR room 184
Lunch will be provided - Seating is limited; pre-registration is required.
Register at www.absciex.com/UFLseminar
AGENDA
12:00 PM Lunch & Registration
12:30 PM Complete Workflow Solutions for PeptideQuantitation Using Accurate Mass Methodologies- This technical workshop will cover targeted peptidequantitation using high resolution accurate mass tandem mass spectrometry and complete data processingstrategies to enable highly productive assay development of peptides and post-translational modifications in complex biological systems.
1:30 PM Leveraging High Sensitivity Accurate Mass MS/MS at High Speeds-MS/MS-ALL on theTripleTOF 5600 system- MS/MS-ALL is a technique using information independent MS/MS acquisition of all precursor ions in a Q1-resolved window sweep through a given mass range. It allows for limitless interrogation of allanalytes from a simple, development-free acquisition technique.
2:15 PM Q&A, Adjourn
Proteomics Seminar Series: Eksigent Seminar - Simplifying NanoLC Workflows and Increasing Throughput Using Chip-Based Columns
Speaker: Dr.Khaled Mriziq, Senior Applications Specialist, Eksigent
Date: Thursday, January 19, 2012
Time: 11:00 AM to 1:00 PM
Location: Cancer Genetics Research Building, ICBR room 184
Lunch will be provided - Seating is limited; pre-registration is required.
Register at www.absciex.com/eksigent
Eksigent, part of AB SCIEX, has been developing nano- and micro-LC platforms for analytical chemists in academic andbiopharma research for over 10 years.
This presentation will focus on:
· State-of-the-artnanoLC workflows fornanoflow proteomics (LC/MS)
· Applications ofcHiPLC for robust, reproducible nanoLC/MS
· Features ofmicrofabricatedcHiPLC columns and traps
· New micro scalecHiPLC size for increased throughputnanoLC with potential for increased depth of coverage
Eksigent Host: Jay Kyne (919) 414-5695
* Food or beverages served within ICBR are not intended for use by persons employed by any UF department that prohibits employees from receiving vendor provided refreshments. ICBR will not accept responsibility or liability to check individuals for compliance.