Resource for Informational Updates
2009 » Jan | Feb | Mar | Apr | May | Jun | Jul | Aug | Sep | Oct | Nov | Dec
ICBR Presents Dharmacon Seminars on RNAi and miRNA
RNAi Screening: Steps towads pathway analysis- Kirsteen MacLean, Ph.D. (Application Scientist for Genomics)
Date: June 30th
Time: 11:30-12:30
Location: CGRC room 184
RSVP: tanya.beaver@thermofisher.com
Topics discussed in this seminar:
• Introduction to RNAi as a tool for screening/functional studies
• Strategies to ensure the function and specificity of siRNA sequences
• Screening using shRNAmir-based RNAi
• Systematic and nonsystematic approaches to reduce error in RNAi screening
miRNA expression profiling and the use of miRIDAN miRNA Mimics and Inhibitors-Tools for understanding miRNA biology and biomarker discovery- Kirsteen MacLean, Ph.D. (Application Scientist for Genomics)
Date: June 30th
Time: 10:00-11:00
Location: CGRC room 184
RSVP: tanya.beaver@thermofisher.com
Topics discussed in this seminar:
MicroRNAs (miRNAs) operate as key regulators of gene expression by repressing mRNA translation or targeting transcripts for degradation. Recent reports on the role of miRNAs in development and differentiation suggest novel opportunities to employ synthetic miRNA mimics and inhibitors as molecular tools in a variety of applications. These applications include miRNA expression profiling for prognostic or diagnostic indicators of normal or disease states or screening with synthetic miRNA mimics and inhibitors to elucidate key regulators of specific biological pathways. In this talk we’ll review examples of miRNA-based studies to highlight the utility of these tools in basic biology and drug discovery platforms.
ICBR Presents Isilon Lunch and Learn
When: Tuesday, June 2, 2009
Where: Cancer Genetics Research Building Room 184
Lunch Provided, Please RSVP to Sydney Carriere
C 386-314-9493 or email Sydney.carriere@isilon.com
NOTE: This seminar is also provided online, details for joining are below.
Isilon Scale Out NAS Solutions Accelerate Critical Applications,
Workflows, and Processes
Isilon's innovative scale-out NAS storage solutions are designed to help
enterprises overcome the most pressing storage challenges facing their
businesses. From streamlining content production, to enhancing the
reliability of file services, to supporting key disaster recovery
initiatives - clustered storage solutions from Isilon combine the
OneFS(r) operating system with the latest advances in industry-standard
hardware to enable customers to accelerate their critical applications,
workflows and processes, while reducing capital and operating
expenditures.
Isilon provides:
* The first and only truly symmetrical clustered storage
architecture
* Unmatched aggregate throughput from a single file system, over 30
GB/second
* Single stream performance of 400 MB/second and concurrent
performance of 700 MB/second
* 60-second scaling of both performance and capacity
* Powerful, easy-to-use web-based management capabilities
* A solution that can scale from 6 TB-3.45 PB with greater than 80%
utilization (GUARANTEED)- all in a single file system
Sydney Carriere invites you to attend this online meeting.
Topic: Introduction to Isilon Scale Out NAS
Date: Tuesday, June 2, 2009
Time: 11:00 am, Eastern Daylight Time (GMT -04:00, New York)
Meeting Number: 331 659 488
Meeting Password: isilon
Please click the link below to see more information, or to join the meeting.
-------------------------------------------------------
To join the online meeting (Now from iPhones too!)
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1. Go to https://isilon.webex.com/isilon/j.php?ED=110379087&UID=1001395977&PW=d4005a474044494a
2. Enter your name and email address.
3. Enter the meeting password: isilon
4. Click "Join Now".
ICBR Presents Applied Biosystems Real-Time PCR Seminars and Lunch
When: Tuesday, June 9, 2009
Where: Cancer Genetics Research Building Room 184
Lunch Provided, Please RSVP to andria.doty@lifetech.com.
Time: 9:00m-12:00pm with a wet demonstration of Taqman miRNA card analysis and data interpretation in the afternoon from 2-5:00pm.
There will be a series of three seminars as follows:
9:00am-QPCR Workflow through Analysis: Dave Chappel FAS
10:00am-Single Cell Profiling with Applied Biosystems Taqman Array (TLDA) Technology: Eric Blair Gene Expression ASC
11:00am-Taqman Genotyping: New Tools to Help you Capture the Whole Picture from Your Research: Brent Anderson Genotyping ASC
Information about meeting our Gene Expression Applications Consultant, our SNP Genotyping Applications Consultant please contact Andria Doty at 352-275-2735 or andria.doty@appliedbiosystems.com
Also there will be an RSVP full catered Lunch for 50 researchers that afternoon with an interest in this technology. There will be free gifts and a full miRNA workflow analysis guidebook for any registered participant which is a great technical resource guide for your laboratory. Available only through the RSVP lunch on June 9th 2009.
Note re Individual Meetings: Applied Biosystems is happy to set up individual appointments to further discuss upcoming research projects and how the technology they can offer to UF investigators can be implemented. The meetings can occur the next day following the seminar on June 10th 2009. Please contact either Eric Blair, MBA, Gene Expression Applications Consultant, Applied Biosystems at 301-801-8004 or by e-mail, at Eric.Blair@appliedbiosystems.com , Brent Anderson, Genotyping Applications Consultant at Brent.Anderson@appliedbiosystems.com or you may contact Andria Doty, ABI Molecular Biology Sales Representative at andria.doty@appliedbiosystems.com for information on scheduling a consultation with Eric Blair, or Brent Anderson to discuss the technology and research tools. This will be the only day they will be available for consultations so please do not hesitate to make an appointment to speak to them about your upcoming research ideas and needs.
Abstract:
Single Cell Profiling With Applied Biosystems TaqMan® Array (TLDA) Technology
The ability to analyze gene expression down to the single cell level is paramount in cancer research where only a limited number of cells such as cancer stem cells are often responsible for initiating and maintaining the disease. This talk will discuss high throughput, high sensitivity easy to implement Applied Biosystems TaqMan® Array (TLDA) solutions currently used at NCI for Stem Cell and/or custom panels of 12-384 genes to quantitate gene signatures down to very low cell sample populations (1-50 cells).
We will demonstrate techniques that can be employed for quantitating small cell populations from cell sorting, laser capture microdissection (LCM) or other selection procedures. We will discuss: 1) Techniques necessary to reproducibly analyze the expression of any targeted mRNA and microRNA gene expression pathway with TaqMan® Arrays; 2) We will highlight processes for the entire workflow from RNA extraction to quantitation of mRNA/microRNA using the TaqMan® Array to automated data analysis with Integromics Statminer software. Time will be given for open discussion.
TaqMan Genotyping: New Tools to Help You Capture the Whole Picture from Your Research.
Abstract: Single Nucleotide Polymorphisms (SNPs) and Copy Number Variants (CNVs) are becoming increasingly more important in genomic research. In order to effectively measure the significance of these biomarkers, researchers are looking to more efficient ways of understanding the significance that SNPs and CNVs play in their research. In this talk, we will discuss two industry leading tools to enable investigators to effectively answer those questions, specifically addressing mid density, high throughput genotyping, as well as, Copy Number Variation assays.
ICBR Presents Roche Real-Time PCR Lunch and Learn with Demo
When: Tuesday, May 19, 2009
Where: Cancer Genetics Research Building Room 184
Presenter: John Mehew, M.A. Roche Applied Science
Free of charge and lunch will be provided!
Please R.S.V.P. to: norton@biotech.ufl.edu
11:30 am to 12:00 pm- Lunch
12:00 pm to 1:00 pm- LightCycler480 Presentation
1:00 pm to 3:00 pm- Wet lab: set up real-time PCR
3:00 pm to 4:30 pm- Final results and conclusion
Participants will have educational lectures and hands-on opportunity during the wet lab.
For more information contact John Mehew at john.mehew@roche.com
or Sharon Norton at norton@biotech.ufl.edu
Cellomics Spring Seminar Series 2009
Mark Your Calendars!
All seminars occur from 12:00-1:30 pm in the Cancer and Genetics Research Complex, Room 184.
Lunch is included and no RSVP is required.
Wednesday, 04/22/09
"Label free technology with CellKey and Imaging with ImageXpress Micro"
Sponsored by Molecular Devices
Demo may follow seminarWednesday, 04/29/09
"AmpliGrid single cell PCR system"
Sponsored by Olympus-Advalytix
All dayWednesday, 05/06/09
"OctetQK"
Sponsored by ForteBio,Rick Gordon
Wednesday, 05/13/09
"ChIP"
Sponsored by Millipore,Chijioke Mbanu
Wednesday, 05/20/09
"PerkinElmer Operetta: Ease of Use and Flexibility of High Content Cellular Imaging and Analysis"
Sponsored by Perkin Elmer,Aaron Risinger
Microscopy Workshops , Feb. 17-18, 2009
Zeiss on your Campus is a series of lectures and hands-on training sessions focused on the practical use of Advanced Imaging Systems.
Location: University of Florida, CGRC 189
Date: 2/17-2/18, 2009
Time: 10am to 5 pm
Registration: www.zeiss.com/signup
Please see 2nd page of the flyer for schedule.
Advanced Technologies for Protein and Peptide Analysis, Feb. 18, 2009
Location: University of Florida
Cancer & Genetics Research Center (ICBR)
Room 184
Gainesville, FL
8:30 am Registration
9:00 am Improving Bioseparations, Utilizing UPLC® Technology in Biochemistry
Ann Gray, Waters Corporation
Separations of complicated sets of molecules derived from biological systems present particularly challenging separations problems. The new separation technique of UPLC has the potential to contribute to improving such separations in terms of resolution, sensitivity and speed. Peptide mapping is an important analytical technique used for protein characterization. UPLC offers significantly better resolution than can be obtained by HPLC, enabling confidence in detection and identification of minor peptide variants. UPLC separation chemistries possess unique chemical properties that extend their use for glycopeptide analyses and for use with LC/MS. For amino acid analysis a completely integrated system solution ensures accurate and precise qualitative and quantitative analyses for samples. UPLC oligonucleotide separation methods are based on the principles of ion-pair reversed phase chemistries.
10:00 am Break
10:15 am Enabling Sample Preparation for Life Science Applications
Kevin Clodfelter, Waters Corporation
Successful LC/MS analysis is a function of many factors, one of which is a robust sample preparation platform. New approaches to dealing with complex proteomic samples in challenging application areas such as phosphoproteomics and glycan analysis have been developed.
10:45 am High Performance Routine Characterization of Proteins Using LC/MS/MS Solutions
Larry Abbey, Waters Corporation
LC/MS has been an enabling technology for characterizing proteins including peptide mapping and intact mass analysis. High resolution mass spectrometry coupled with high resolution, high sensitivity UPLC enables methods for protein characterization. These methods are employed for studying the structural changes associated with protein production, formulation and storage. Peptide mapping is an important analytical technique used for protein characterization. Waters High Definition (HD) Proteomics Strategies use many innovative technologies to comprehensively characterize and visualize peptide and protein characteristics. Our visualization of proteins routinely includes characterization of
post-translational modifications, protein isoforms, protein sub-cellular localization, protein
turnover/synthesis, protein structure/folding, protein interactions, and stoichiometry.
For additional information contact Craig_Langway@waters.com, 800-252-4752 Ext. 6771
Register on-line at: www.waters.com/ATPPA