Overview
Divisions » Bioinformatics | Cellomics | Genomics | Proteomics
The Cellomics division of ICBR is devoted to assisting investigators in the study of cell structure, function and generation and application of cellular products.
The ICBR Hybridoma Research Laboratory's (Linda Green) primary service is the development of new monoclonal antibodies. Monoclonal antibodies are used in many areas of basic scientific research, industry, human and animal medicine and agriculture. Monoclonal antibodies can be generated to almost any substance (toxins, drugs, blood proteins, cancer cells, viruses, hormones, environmental pollutants, food products, metals, plant materials). Monoclonal antibodies are routinely used to create sensitive tests for detection and quantitation of various substances. These reagents are also routinely used in other applications such as flow cytometry and electron microscopy.
Assistance is available for antigen and immunoassay design. The Hybridoma Research Laboratory will carry out all phases of hybridoma development: immunizing mice, fusions, screening (ELISA and western blotting) single cell cloning, mycoplasma testing and isotype determination. Other services include high density in vitro antibody production, purification and labeling of monoclonal antibodies. UF investigators may contract with this laboratory for selection of human scFv reagents using phage libraries that have been licensed from the developers. Training opportunities in mammalian cell culture techniques and antibody applications are offered in collaboration with the ICBR Education and Training Lab.
The ICBR Flow Cytometry Research Laboratory (Neal Benson, MS) offers services that are used for detecting cell surface or intracellular proteins, measuring apoptosis, cell cycle, cellular physiology, and have applications in both research and clinical medicine. The staff is available for consultation in experimental design and technical issues such as sample preparation. We can provide training for users that wish to learn the full operation of the instrumentation for self-service analysis, or we can analyze samples for you. Our most advanced instrumentation allows for up to 9 color experiments, and the cell sorter can be used to isolate specific populations of cells either in bulk or cloned as single cells into 96 well plates. This is done under sterile conditions so that the recovered cells can be put back into culture for further experimentation or for single cell PCR.
The Electron Microscopy & Bioimaging Laboratory (Byung-Ho Kang, PhD) provides basic services in both transmission and scanning electron microscopy. We can provide complete or partial service on a sample. These services include thin-section EM, negative staining, replica formation, immunolabeling, and preparation for scanning EM. This laboratory offers an informal training program for those who want to learn all or part of the technology associated with EM studies. This includes operation of the microscopes. We also provide access to a fluorescent microscope for any basic optical microscopy studies. All instruments are equipped with high resolution digital cameras. Data is provided via an FTP site or it can be provided to users on CD or DVD.
Direct Cellomics Division Inquiries to Linda Green…