Cellomics

Core Laboratories

Electron Microscopy and Bioimaging

The Electron Microscopy and Bioimaging core supports researchers in visualizing structures of microscopic samples by carrying out imaging projects and user training to facilitate solving research questions and promote acquisition of grants. Major service categories include: transmission and scanning electron microscopy, confocal laser scanning and epiflourescence microscopy, preparation of samples for microscopic analyses, and training users to operate microscopes and ancillary equipment.

To contact Electron Microscopy and Bioimaging, e-mail ICBR-ElectronMicroscopy@ad.ufl.edu.

Flow Cytometry

The Flow Cytometry core assists investigators in quantitatively identifying cells and determining their role in essential biological processes. Major service categories include: flow cytometry analyses (including but not limited to immune cell analysis), apoptotic cell quantitation, cell cycle and growth kinetic studies, cell sorting to enrich for subpopulations of live cells, confocal microscopy on live cells, and experimental design assistance. Samples for analysis must be prepared by the investigator’s staff, but core staff members can often provide advice and protocols. Consistent customers can be trained to use all of the core’s instruments EXCEPT cell sorters.

To contact Flow Cytometry, e-mail ICBR-FlowCytometry@ad.ufl.edu.

Hybridoma

The Hybridoma core develops new mouse monoclonal antibodies for investigators to use in basic and applied research. Major service categories include: immunization of mice, cell fusion, cell cloning, screening by ELISA and Western blot, monoclonal antibody production, purification, and labeling, monoclonal antibody analyses such as antibody/antigen affinity measurements, epitope binning, and identification of matched antibody pairs. Customer training on select self service instruments is also offered.

To contact Hybridoma, e-mail ICBR-Hybridoma@ad.ufl.edu.