Cellomics

The Cellomics division is devoted to assisting investigators in the study of cell structure, function and generation and application of cellular products.
 

Core Laboratories

Electron Microscopy and Bioimaging

The Electron Microscopy and Bioimaging core supports researchers in visualizing structures of microscopic samples by carrying out imaging projects and user training to facilitate solving research questions and promote acquisition of grants. Major service categories include: transmission and scanning electron microscopy, confocal laser scanning and epiflourescence microscopy, preparation of samples for microscopic analyses, and training users to operate microscopes and ancillary equipment.

To contact Electron Microscopy and Bioimaging, e-mail ICBR-ElectronMicroscopy@ad.ufl.edu.

Flow Cytometry

The Flow Cytometry core provides a variety of tools and expertise for cellular measurements. The laboratory incorporates numerous flow cytometers, from simple entry-level devices to high-end 5-laser, 16-parameter instruments to analyze or sort cells. In addition, the laboratory offers a laser scanning confocal microscope. The microscope is well equipped with a variety of excitation lines from near UV to red and can collect 3 to 4 emission colors (depending on dye combinations), with viable cell time lapse and extensive computed parameter capabilities, including FRET, FRAP, image stitching, and 3D reconstruction.

The laboratory offers two sites:  the Cancer/Genetics Research Complex and a satellite laboratory at the McKnight Brain Institute. Consultation is available for planning cytometric assays and carrying out the analytical portion of the assay, including analysis of the resulting data. Customer training in analytical self service instrument operation is available.

To contact Flow Cytometry, e-mail ICBR-FlowCytometry@ad.ufl.edu.

Hybridoma

The Hybridoma core develops new mouse monoclonal antibodies for investigators to use in basic and applied research. Major service categories include: immunization of mice, cell fusion, cell cloning, screening by ELISA and Western blot, monoclonal antibody production, purification, and labeling, monoclonal antibody analyses such as antibody/antigen affinity measurements, epitope binning, and identification of matched antibody pairs. Customer training on select self service instruments is also offered.

To contact Hybridoma, e-mail ICBR-Hybridoma@ad.ufl.edu.