Genomics

The mission of the Genomics division is to provide life scientists affiliated with the University of Florida as well as other academic and commercial entities, with services and expertise using the most current instrumentation for specialized genetic data acquisition at a reasonable cost. We are equipped to assist investigators at any stage in their research, from experimental design and sampling strategies though publication.

Resources

 

Core Laboratories

Gene Expression

The Gene Expression core provides state-of-the-art technical services and consultation on Affymetrix and Agilent gene expression arrays and miRNA arrays, RNA-seq libraries, sequence capture libraries, SOLiD SAGE barcoding, and up-to-date gene expression analysis methods, allowing scientists to be on the cutting edge of research.

To contact Gene Expression, e-mail ICBR-GeneExpression@ad.ufl.edu.

Genotyping

The Genotyping core provides a variety of genotyping services including fragment analysis using the Advance FS96 and AB3730 (96 capillary technology), development of microsatellite libraries using 454 sequence data, Illumina SNP and gene expression data, and genotyping using mouse tails or ear punches. The core has extensive experience in applying stat of the art molecular technology to studies in population, conservation, ecological, and evolutionary biology.

To contact Genotyping, e-mail ICBR-Genotyping@ad.ufl.edu.

NextGen DNA Sequencing

The NextGen DNA Sequencing core provides quality, massively parallel, high throughput sequencing data using the most current instrumentation, such as PacBio, 454, Ion Torrent, and Illumina, covering a broad range of sequencing applications for biological research. Customers are encouraged to consult with core staff for the purpose of tailoring data acquisition to specific research questions.

To contact NextGen DNA Sequencing, e-mail ICBR-NextGenSeq@ad.ufl.edu.

Quantitative PCR

The Quantitative PCR core provides researchers access to instruments which support SYBR® or TaqMan™ reagents allowing absolute, relative, relative standard or comparative Ct quantification. Applications include quantitative gene expression analysis, pathogen detection/quantification, allelic discrimination (SNP detection), and mutation screening. The core provides self service training and service as well as technician-assisted service to researchers and lab staff members.

To contact Quantitative PCR, e-mail ICBR-qPCR@ad.ufl.edu.

Sanger Sequencing

The Sanger Sequencing core provides capillary-based automated DNA sequencing. One-lane sequencing processes, which use four-dye fluorescent labeling methods and a real-time scanning detector, are used. The sequencers automatically perform the electrophoresis, call the bases, and archive the data. Customers are alerted when sample runs are complete and data are available for download.

To contact Sanger Sequencing, e-mail ICBR-SangerSeq@ad.ufl.edu.