The Mass Spectrometry core provides a variety of services related to protein fractionation, qualitative and quantitative mass spectrometry analysis, de novo protein sequencing and protein database searching. Protein sequencing and identification are routine procedures in the core.The core has a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination. To ensure success and maximize productivity, the Mass Spectrometry core offers education, consultation, data processing and reporting, and support of grant application.
To contact Mass Spectrometry, e-mail ICBR-MassSpectrometry@ad.ufl.edu.
The Protein Biomarkers core provides a variety of services related to biomarker discovery, such as protein abundance analysis using gel-based 2 -D Fluorescence Difference Gel Electrophoresis, protein separation using 1-D or regular 2-D gel electrophoresis and Western blotting; 2-D gel-based phosphor protein and glycoprotein profiling. The core also provides protein enrichment and abundant protein depletion from biological fluids and liquid phase preparative IEF to fractionate protein or peptide mixtures.
To contact Protein Biomarkers, e-mail ICBR-ProteinBiomarkers@ad.ufl.edu.