Workshops


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Sequence Analysis: Theories and Applications
Date: TBA

Time: 8:00AM - 5:00PM

Location:
ICBR Room 184-South Wing
Cancer and Genetics Research Complex
Gainesville, FL
Fees:
$150.00 for registered UF students, UF Faculty and UF Staff
$150.00 for all others
Instructor:
Li Liu, MD
Description:
This workshop focuses on how to use public databases and free bioinformatics software to facilitate nucleotide and protein sequence analysis. For most of the software discussed in this workshop, we go over the basic theories, algorithms, and their applications. There is a 2-hour lecture and a 1-hour hands-on practice for each topic we cover. The topics include:
  • Contig Assembly
  • Database Search
  • Sequence Alignment
  • Gene Structure Prediction
  • Gene/Protein Function Prediction
  • Primer Design
  • Cloning in silico, Restriction map, and Gel Simulation (on request)

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Introduction to Mammalian Cell Culture Techniques
Date: 10/14-10/15/2008

Time: 8:30AM - 1:00PM

Location:
ICBR Room 184-South Wing
Cancer and Genetics Research Complex
Gainesville, FL
Fees:
$150.00 for all participants
Instructor:
Linda Green
Description:
Cell culture is one of the most widely used techniques in life sciences. This Cell Culture workshop will cover the basic techniques used for maintaining animal cells in culture: aseptic technique, counting cells, subculturing, cryopreservation (freezing) and thawing. Participants will obtain hands-on training in all techniques listed above. Lecture and discussion sessions will include the techniques mentioned above as well as the following topics: cell culture instrumentation, contamination and optimization of growth conditions.

Sigma-Aldrich is sponsoring this workshop and will be providing refreshments and a guest speaker: George Sitterley, Cell Culture Product Manager.
 

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Flow Cymtometry Core User Training
Date:TBA

Time: 9:00AM-12:00PM

Location:
ICBR Room 184-South Wing
Cancer and Genetics Research Complex
Gainesville, FL
Fees:
No Charge
Instructor:
Neal Benson, MS
Description:
This workshop is designed for new users of the Flow Cytometry Core. It is directed primarily towards those individuals who would like to use our flow cytometers to analyze samples on their own. Composed of 6 contact hours over two days, the topics covered will be:
    Day 1:
  • Flow Cytometry Core policies and services
  • Introduction to instrument and software features
  • Basic principles of flow cytometry and instrument operation
  • Outline of common applications and fluorescent stains
    • Day 2:
  • Working with the Macintosh operating system
  • Utilizing Cell Quest software to collect and plot data
  • Flow cytometry demonstration
  • Startup and shutdown of the cytometer

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Molecular Markers
Date: 12/08-12/2008

Time: 8:00AM - 5:00PM

Location:
ICBR Room 184-South Wing
Cancer and Genetics Research Complex
Gainesville, FL
Fees:
$500.00 for registered UF students, postdocs and residents
$900.00 for all others
Instructor:
The main instructor is Dan Brazeau. Supporting instructor is Ginger Clark, MS.
Description:
This intensive laboratory and lecture workshop allows participants to build and screen a microsatellite-enriched, plasmid library using the genomic DNA of their choice. Repetitive probes immobilized on magnetic particles are used to capture microsatellite-containing fragments from genomic DNA digests. A session is dedicated to DNA fragment analysis (GeneScan and Genotyper software). Other experiments in the workshop include procedures for library screening, random amplification of polymorphic DNA (RAPD), Amplification Fragment Length Polymorphisms (AFLP) and allozyme analysis. Complementary lecture topics focus on the use of microsatellites in determining genetic relationships, molecular probes for analysis of population structures, and applications of RAPDs in population genetics.

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2D Gel Electrophoresis
Date: By Arrangement, please contact Sharon Norton

Time: 3 day course

Class Size: 5 max

Location:
ICBR Room 184-South Wing
Cancer and Genetics Research Complex
Gainesville, FL
Fees:
$300.00 for all participants
Instructor:
Marjorie Chow, MS
Description:
The participants in this 3-day laboratory and lecture workshop will use E. coli protein extracts to learn and practice state-of-the-art 2-dimensional electrophoretic techniques for protein analysis. Workshop protocols have been designed to precede mass spectrometric analysis and protein identification. Researchers wanting to use their own protein samples in the workshop will be allowed to do so, only if previous arrangements have been made with workshop instructor at least one month prior to the event. For Your Own Sample Preparation, please contact Marjorie Chow (352-273-8059) at least one month before workshop.
    Course Content:
    Hands-on Methodologies for 2-D PAGE
  • Sample preparation (optional)
    • Tissue or cultured cell protein extraction
    • Protein extract clean up
    • Protein determination
  • First and second dimension gel electrophoresis
  • Detection methods using either Colloidal Coomassie blue and Sypro Ruby stains
    • Review and Discussion on
  • Sample preparation
  • 2-D gel electrophoresis theory
  • 2-D gel electrophoresis reagents
  • Trouble shooting
  • 2-D differential gel electrophoresis (DIGE)
    • DIGE demonstration
  • CyDye labeling
  • Image analysis with Typhoon phosphoimager

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Proteomics
Date: TBA

Time: 9:00AM - 4:00PM

Location:
ICBR Room 184-South Wing
Cancer and Genetics Research Complex
Gainesville, FL
Fees:
$300.00 for registered UF students, postdocs and residents
$400.00 for all others
Instructor:
TBA
Description:
Mass spectrometric-based methodologies have become increasingly important for a wide variety of proteomics applications. Coupled with high-resolution separation techniques such as gel electrophoresis and liquid chromatography, mass spectrometry allows for rapid protein identification and characterization with high sensitivity and accuracy. This workshop will include an introduction to modern proteomics experiments such as protein quantification by differential fluorescence gel electrophoresis (DIGE) followed by mass spectrometric analysis to determine protein identity. In addition, the use of mass spectrometry and bioinformatics tools to identify sites of posttranslational modification sites will be covered.

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