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Advances in Luminescent & Fluorescent Technologies in Accelerate Research
October 10, 2012 @ 12:00 PM - 1:00 PM
Luminescent- and fluorescent -based assays are powerful research tools and integral components of many research projects. Recent laboratory-directed evolution of a deep sea shrimp luciferase and its natural substrate led to the development of a new luciferase reporter system, which is ~100-fold brighter than either firefly or Renilla luciferase both in vitro and in cells. Additional research led to the development of a novel cell impermeable, non-toxic, fluorescent DNA dye that is a powerful tool for assaying cell cytoxicity. This seminar will describe the development of the new luciferase assay systsem, the three reporter genes available, and the application of this technology to gene transcription and BRET-based studies such as protein-protein interaction assays and receptor-ligand interactions. Finally, an overview of the DNA dye-based cytotoxicity assay will be presented including a comparison to enzyme-based cell viability assays and multiplexing with other assays to better define mechanisms of cell death.
Presented by: Robert M. Brazas, Ph.D., Promega Corporation
Event Flyer: UF NanoLuc CellTox Green Seminar Flyer 2012
Lunch will be provided.
Please RSVP to: Kara chamberlain, Ph.D., Sr. Field Applications Specialist, 850-590-4844, firstname.lastname@example.org