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AmpliSeq Cancer Panel
July 10, 2012 @ 10:00 AM - 2:00 PM
Life Technologies Ion AmpliSeq Cancer Panel allows translational and clinical researchers to fast-track oncology research by focusing directly on “hot spot” regions (739 COSMIC mutations in 604 loci) from 46 oncogenes and tumor suppressor genes with emphasis on variants in the KRAS, BRAF, and EGFR genes for detecting somatic mutations in archived cancer samples. Designed with input from premier oncologists and cancer researchers, the AmpliSeq Cancer Panel allows accurate and affordable detection of low-frequency mutations. Features of the AmpliSeq Cancer Panel include:
- Sample input requirement of only 10 ng of DNA
- Ability to sequence challenging samples like FFPE tissue
- Single-day workflow that requires significantly less time to obtain sequence data than protocols on other platforms
- Optional barcoding that allows multiplexing for large studies.
Using the AmpliSeq technology, the ICBR NextGen Sequencing lab recently sequenced 4 samples from the Moffitt Cancer Center. DNA was extracted from FFPE blocks or whole blood, and barcoded AmpliSeq libraries were prepared from 10 ng of input DNA for each of the 4 samples. Libraries from the 4 tumor samples were pooled and run together on an Ion Torrent 316 chip. DNA sequence data were available by the end of the day!
Data from the AmpliSeq Cancer Panel can be transferred to the Torrent Browser and aligned to the hotspot BED file to generate a variant calling report. Additionally, raw data can be downloaded as fastq or sff files for analysis in 3rd party analysis packages. Data also can be pushed to Ion Reporter, a cloud hosted software designed for variant calling and annotation.