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BioNano Genomics: Illuminating the Dark Matter
November 30, 2016 @ 10:00 AM - 11:00 AM
Illuminating the Dark Matter:
De Novo Assembly and Mapping of the Structural Variation of the Genome.
The majority of complex genomes are made up of repetitive and regulatory elements, yet our current analysis of the genome focuses on single nucleotide changes and exome sequencing. The “Dark Matter” that controls most of our genome is largely ignored, because short read sequencing is unable to map these highly repetitive sequences.
BioNano Genomics’ Irys instruments use nanochannel arrays to linearize and image megabase size single DNA molecules. This extremely long read data can elucidate genome-wide complex structural variation like balanced/unbalanced translocations, inversions, and large indels. Our de novo genome maps can identify and phase CNVs, resolve complex repetitive regions, scaffold short and long-read NGS contigs to create better-than-reference assemblies, and resolve complex repetitive regions.
Applications range from bacterial engineering to building reference genomes for complex polyploid plants all the way to clinical use. We will touch on long range phasing of methylation patterns, and analysis of chromatin structure.
For more information, please see our flyer: UF ICBR BioNano Seminar flyer