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Cellomics Seminar Series 2012: Union Biometrica, Inc.
June 6, 2012 @ 12:00 PM - 1:00 PM
Flow Cytometry for Analysis and Sorting of Large Objects
Rock Pulak, Ph.D., Global Director of Life Science Technologies
Many objects are too large or fragile for conventional flow cytometry. Union Biometrica, known for theCOPAS instruments, introduces theBioSorter instrument for the automated analysis and sorting of large (10-1,500 micron) objects at rates of 10-500 objects/second. Using object size, optical density and fluorescence intensity as sorting criteria, selected objects can be dispensed in multi-well plates or bulk receptacles. A gentle pneumatic sorting mechanism avoids harming sensitive objects thereby making the instrument suitable for live biological materials or sensitive chemistries. This includes Fragile Large Cells & Cell Clusters such as hepatocytes, adipocytes, cardiomyocytes, kidney tubular cells,embryoid bodies, pancreatic islets. Other suitable samples are small model organisms such as all stages of C.elegans, Drosophila eggs, embryos and larvae, andZebrafish eggs, embryos and the early larval stages. Finally, small plant seeds, like Arabidopsis, and plant pollen, as well as beads andmicrocarriers of various sizes and shapes are all acceptable samples for analysis and sorting.