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Cellomics Seminar Series 2013: DNA Methylation & Cell Line Authentication from Promega
June 5, 2013 @ 12:00 PM - 1:00 PM
“Hot Topics: Mapping DNA Methylation with Bisulfite Conversion & Cell Line Authentication”
Speaker: Robert M. Brazas, PhD
This seminar will cover two important topics in molecular biology: (1) mapping DNA methylation through the use of bisulfite conversion and (2) the growing need to verify the identity of cell lines used during cell-based experimentation. DNA methylation is a key epigenetic mechanism used by cells to regulate gene expression, and mapping methylation patterns is a primary focus of many life science researchers. Bisulfite conversion combined with various downstream assays is a considered the gold standard for DNA methylation analysis, and the first part of this seminar will include a brief overview of epigenetics/DNA methylation followed by the challenges of bisulfite conversion and guidelines for overcoming those challenges while also optimizing the conversion process. As more contaminated cell lines are identified often resulting in the invalidation of data, the need for cell line authentication is rapidly increasing and journals are beginning to strongly recommend it as part of the paper submission process. In the second half of this seminar, we will discuss the cell line authentication guidelines put forth by ANSI. We will also introduce the basic principals of short tandem repeat (STR) profiling, the favored molecular method to verify cell lines, and include a detailed discussion of STR profiling workflow.
Lunch will be provided. Registration is not required for this event.