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Cellomics Seminar Series 2013: Improving Protein Detection from EMD Millipore Corporation

May 16, 2013 @ 12:00 PM - 1:00 PM

“Novel Approaches for Improving Protein Detection: Quantification with FTIR and Multiple Target Analysis with Multiplex Technology”

Speaker: Ramsey McIntire, PhD, Protein Specialist, Southeast US

Cytokines, hormones, growth factors, and signaling proteins in biological fluids/cells/tissues are critical factors in numerous processes in healthy and disease states, and in most models multiple pathways are involved in simultaneously producing a cellular response to disease or experimental stimulation. In order to assess multiple targets and gain a more complete picture of the pathways involved in a physiological process, researchers need to:

  1. Reliably quantify total protein from limited sample volumes. Traditional methods for quantification of total protein in complex samples are time-consuming and potentially inaccurate due to the presence of contaminating lipids, detergents, and reducing agents.
  2. Measure dozens of targets, both secreted factors and intracellular signaling components, within the same sample. Target-specific detection by ELISA and Western blotting are costly, time-consuming and require large volumes of valuable samples when measuring multiple analytes.

Please join EMD Millipore for a highly technical seminar describing two novel approaches for protein detection, the Direct Detect FTIR system and Multiplexing assays on the Luminex platform. The presentation is suitable for every level of scientist involved in protein research. Both of these technologies enable researchers to more fully understand the complexities of their cell/tissue samples by more accurately quantifying proteins and lipids, saving precious sample volume, and generating dozens of data points from a single biological sample.

Lunch will be provided. Registration is not required for this event.

Food or beverages served within ICBR are not intended for use by persons employed by any UF departments that prohibit employees from receiving vendor provided refreshments. ICBR will not accept responsibility or liability to check individuals for compliance.

Details

Date:
May 16, 2013
Time:
12:00 PM - 1:00 PM

Venue

CGRC Room 451 A/B
2033 Mowry Road
Gainesville, FL 32610 United States
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Organizer

ICBR
Phone:
(352) 273-8030
Email:
ICBR-ContactUs@ad.ufl.edu
Website:
www.biotech.ufl.edu