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Cellomics Seminar Series 2014: Transfection Methods and Automated Fluorescence Microscopy with LED
March 19, 2014 @ 12:00 PM - 1:00 PM
Speakers: Scott Jamison, Life Technologies Brand, Thermo Fisher Scientific & Scott McGinnis, Advanced Microscopy Group, Thermo Fisher Scientific
Life Technologies provides a complete selection of transfection reagents designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures. In addition, Lifetech has the Neon™ Transfection System, a novel, benchtop electroporation device that employs an electroporation technology, using the pipette tip as an electroporation chamber to efficiently deliver nucleic acids, proteins, and siRNA into all mammalian cell types, including primary and stem cells, with a high cell survival rate.
From car headlights to football stadiums, LED light sources that use little electricity and last for tens of thousands of hours are becoming the standard for green lighting around the world. With more power and increased stability these sources can now be integrated directly into microscope systems as excitation sources.
LED’s eliminate the damaging UV/IR wavelengths and heat of traditional light sources making them ideal for live cell microscopy. Their added ability to be driven electronically via software also creates a microscope light source that is only on when it is needed, saving valuable bulb life and reducing sample exposure to potentially damaging excitation wavelengths.
This talk will discuss how the advances in this technology are being implemented in the next generation of automated fluorescence microscopes.
Lunch will be provided to attendees.
Food or beverages served within ICBR are not intended for use by persons employed by any UF departments that prohibit employees from receiving vendor provided refreshments. ICBR will not accept responsibility or liability to check individuals for compliance.