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July 7, 2016 @ 11:30 AM - 12:30 PM
“NGS Library Preperation Technologies for Whole Transcriptome Sequencing Versus Expression Profiling”
Join us this July 7, for a special seminar with Lexogen at UF ICBR. The event will take place from 11:30am to 12:30pm in the room 451.
Lexogen has developed different approaches for whole transcriptome sequencing and gene expression profiling: SENSE and QuantSeq.
The SENSE library preparation kit generates ready-to-sequence libraries from low amounts of total RNA in less than 5 hours. SENSE libraries are significantly more strand-specific (>99.9 %) than common alternatives, thus allowing the most accurate mapping of reads to their corresponding strand on the genome. This enables the confident discovery and quantification of antisense transcripts and overlapping genes.
The QuantSeq kit provides an easy protocol to generate highly strand-specific NGS libraries close to the 3’ end of polyadenylated RNAs. In contrast to whole transcriptome sequencing, only one sequence tag per mRNA is generated. Mapped reads can simply be counted without the need for an annotation based statistical model, and typically a 10-fold lower read depth is required than with standard mRNA-Seq. QuantSeq is superior in differential expression analyses due to its low false positive rate while having the same transcript detection sensitivity as mRNA-Seq. QuantSeq libraries ca be multiplexed up to 96 samples per lane, thereby reducing sequencing cost.