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Seminar: DEPArray™ Digital Sorting
January 13, 2016 @ 12:00 PM - 1:00 PM
Digital Sorting of 100% Pure Cells to Resolve Heterogeneity on Low Input and Low Cellularity Samples
Speaker: Raimo Tanzi, PhD & Carl Woodham, PhD, Silicon Biosystems
Cellular heterogeneity is a problem in science. This heterogeneity limits the effectiveness of downstream molecular analysis, by limiting the usefulness of data collected. The introduction of digital analytical methods, like NGS and dPCR, has helped manage heterogeneity through deeper resolution, but has not provided the clarity that can only be seen in a homogeneous sample.
DEPArray™technology enables the identification and sorting of pure cell populations from a heterogeneous sample. This semiconductor based technology uses dielectrophoresis (DEP) to bind single cells to the electrical charge of single microelectrode on a complementary metal-oxide semiconductor (CMOS) chip and leverages the precision of semiconductor controlled devices for sorting cells in absolute purity and from minute samples.
Cells are treated gently inside the DEPArray chip, so they can be sorted for molecular analysis, or cell culture. In oncology the DEPArray technology’s main applications are in the isolation of single Circulating Tumor Cells (CTC) from enriched blood and the isolation of pure tumor, stromal, or TIL cells from FFPE, fine needle aspirations (FNA) or fresh tissues.
Data indicates that the interpretation of underlying genetic alterations in tumor cells becomes much clearer on samples transformed into collections of pure cells, where somatic variants appear undiluted. Loss of heterozygosity (LoH) is readily detectable and copy number variation (CNV) is not smoothed, leading to a high resolution view of tumor biology.
In this seminar, we will present the principles of operation of the technology, its main applications and a demonstration of results on pure cells from FFPE samples with NGS amplicon panels, Whole Exome Sequencing and Low Pass Genome analysis.