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Seminar: Microbial Sequencing with the Pacific Biosciences System
March 28, 2013 @ 2:00 PM - 3:00 PM
Microbial Pathogenesis: Single-molecule Sequencing to Understand Infectious Disease
Presented by Pacific Biosciences and UF Interdisciplinary Center for Biotechnology Research, please join us on March 28 at 2 p.m. Refreshments will be served.
Please RSVP to firstname.lastname@example.org by Tuesday, March 26.
Speaker: Andres Larrea, PhD, Applications Sales Specialist, Pacific Biosciences
This presentation will discuss how finished and complete microbial genome assembly enables correct identification of strain origins and large structural insertions. This is done by highly-accurate and cost-effective de novo sequencing to permit proper functional annotations of genes. Extra-long read lengths and lack of sequencing bias enable researchers to resolve repetitive and difficult regions of the genome. Finally, there is a way to directly detect methylation. We will describe published work that shows why having microbial epigenomes is key to understanding epigenetic control of biochemical function in bacteria and virulence.
Pacific Biosciences Single Molecule Real Time (SMRTTM) DNA sequencing system enables, for the first time, massively parallel, uninterrupted observation of natural DNA synthesis by a DNA polymerase as it occurs. SMRT DNA sequencing technology is based on the observation of a single DNA polymerase molecule working in a continuous, bias-free manner, and without amplification or bisulfite-conversion to detect base modifications.