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Seminar: Single-cell genomic studies
September 23, 2015 @ 10:30 AM - 1:30 PM
Presented by Fluidigm and ICBR
Current molecular studies analyze the average signal from many cells and may mask the true profile of each individual cell. Using single cell genomic applications, researchers are able to identify differences between individual cells in a seemingly homogeneous population and yield new insights with biological and clinical implications. Join us for the following presentation:
- 10:30-11:30: Best practices for cell prep
- Neal Benson, UF ICBR Cytometry scientific director
- Veronica M. Negron-Perez
- Pedja Sekaric
- 11:30-12:30: New applications and publications, including single-cell ATAC-seq and whole exome sequencing from single cells
- Pedja Sekaric
- 12:30: Lunch
- 12:30-1:30: Open App, the new hub for sharing user-developed C1 protocols
Fluidigm has developed an approach to single-cell genomics based on microfluidic technology which enables highly parallel RNA and DNA analysis from individual cells. This allows researchers to rapidly and reliably isolate, verify, extract, reverse transcribe, amplify, and analyze cell activity using just one technology. The available applications are:
- Single-cell messenger RNA (mRNA) sequencing for whole transcriptome analysis
- Targeted gene expression analysis
- Single-cell DNA sequencing for whole exome or targeted sequencing
- Pre-amplification of microRNA species using TaqMan assays
- Epigenomic ATAC-seq chromatin accessibility
Registration is now closed.
Refreshments will be provided to attendees.
Food or beverages served within ICBR are not intended for use by persons employed by any UF departments that prohibit employees from receiving vendor provided refreshments. ICBR will not accept responsibility or liability to check individuals for compliance.