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Realtime PCR
- How much RNA do I need to provide?
- What is the significance of the melt curve analysis?
- What is the turn-around time for performing a new assay?
- How and in what format will I receive my data?
- Whom do I contact with questions about real-time PCR services within the qPCR lab?
Realtime PCR
- How much RNA do I need to provide?
- We need at least 1 ug total RNA to perform the cDNA reaction efficiently. The products of this reaction are typically sufficient to perform 200 Real-Time qPCR reactions. The qPCR lab can process either eukaryotic or prokaryotic samples.
- What is the significance of the melt curve analysis?
- The melt curve analysis is performed as the final step in each assay as a way to detect non-specific products. These products can result from spurious amplification (due to non-specific priming) and/or the formation of primer/dimers. This analysis is a crucial step when using an intercalating dye such as SYBR Green.
- What is the turn-around time for performing a new assay?
- Once primers & probe sets and RNA samples arrive to the qPCR lab, it typically takes seven to twenty business days to perform the needed optimization reactions. The assay is performed in a 96-well format. Data generation is dependent on the number of samples and genes to be assayed. If you do chemistry by your self and only use our instrument to run the plate you will receive the results the same day.
- How and in what format will I receive my data?
- You will receive an email which contains your data from the qPCR lab. All data is returned to the customers in Microsoft office document image and Microsoft Excel format.
- Whom do I contact with questions about real-time PCR services within the qPCR lab?
- Please contact LI Zhang, Ph.D. with any questions.