ICBR Scientists Assist in Effort to Create RNA-seq Standards

Published: September 4th, 2014

Category: All News, Featured News, NextGen DNA Sequencing

A recent paper in Nature Biotechnology explores established methodology and develops new protocols for RNA sequencing on the four most used next generation sequencing instruments. It was undertaken by members of the Association of Biomolecular Resource Facilities (ABRF), the professional society representing bio-molecular research support laboratories across the US and around the world. ICBR scientists helped develop protocols for RNA (cDNA) sequencing by taking advantage of the revolutionary third-generation DNA sequencing technology introduced by Pacific Biosciences, Inc.

The resulting protocol is the basis of the current recommended method for cDNA sequencing using the PacBio RS II instrument. The PacBio RS II sequences DNA with much longer continuous nucleotide read lengths – 10-100 times longer than any other commercially-available DNA sequencing technology – enabling full length cDNA sequencing for genes of 5,000 nucleotides or more.  

Through this technology, scientists can tell a lot more about RNA structure and how diversely functional proteins are made from the same gene. RNA (cDNA) sequencing helps researchers better understand how the sum of approximately 35,000 human genes can create far more than 35,000 unique proteins. PacBio sequencing additionally allows more complete, facile, and faster sequencing of the whole genomes of viruses, bacteria, and higher organisms. ICBR offers sequencing with the PacBio RS II in the NextGen DNA Sequencing core – contact the core to consult on your next project.

If you are a GenomeWeb subscriber, read more about these methodologies at GenomeWeb.


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