Octet QKe, Hybridoma self service instrument has moved to room 171
The Hybridoma core lab Octet QKe self service instrument has moved from room 167 (Proteomics division) to room 171 (Hybridoma Lab).
It allows label-free quantitation of antibodies, proteins, peptides, DNA, and other biomolecules. Additionally, this instrument provides kinetic characterization of biomolecular binding interactions.
UF investigators are using the instrument for experiments that focus on protein/protein interactions, DNA-protein interactions, RNA-protein interactions, as well as for custom quantitative assays currently in development by several research groups.
Previous uses have included work by PhD candidate Jen McGee, who used the Octet to measure IgG in manatee serum and presented her findings in “Use of BioLayer Interferometry for the Quantification of Sirenian IgG” at The Society for Marine Mammalogy 19th Biennial Conference on the Biology of Marine Mammals.
The Octet QKe is available for self service and full-service use. A two hour training session for self service users may be arranged by contacting the Hybridoma Scientific Director, Linda Green, at ICBR-Hybridoma@ad.ufl.edu.