OCTET Qke: Taking Your Research to the Next Level
The UF ICBR Monoclonal Antibody Core offers the OCTET QKe, an instrument based on Bio-layer Interferometry (BLI) technology. With this label-free, biosensor technology, you can measure molecular interactions in real-time.
The OCTET QKE is capable of label-free, microfluidics-free, biomolecule quantitation and kinetic analysis for proteins, nucleic acid, antibodies, peptides, media containing serum, untreated cell culture supernatant and crude cell lysates.
With the OCTET systems, you can survey a number of different interactions rapidly, while obtaining accurate kinetic and affinity information and determining concentrations of your protein of interest in complex sample matrices. In addition, custom quantitative assays may be developed on this instrument, and it can also detect molecules that have a molecular weight of 5.00 kD or higher.
UF investigators have already been able to use the instrument for experiments focusing on protein-protein interactions, DNA-protein interactions, and RNA-protein interactions.
Octet Service Delivery
There are several modes that customers can choose from for Octet service in the Monoclonal Antibody Core:
- Self service: customer pays an instrument fee based on elapsed time the instrument is used (this will help pay for the service contract) plus customer pays for the cost of expendables (sensors, sensor plate, sample plate, buffers, etc.).
- Full service: customer provides the samples and core staff run the experiments.
The ICBR Monoclonal Antibody is happy to offer all customers a free consultation to explore their particular needs. Additionally, the core offers a complete suite of monoclonal antibody development services, including, but not limited to monoclonal antibody production, quantitation and purification. If you have further questions or would like to schedule a consultation, please contact ICBR-MonoclonalAntibody@ad.ufl.edu.