Orbitrap Mass Spec added to Proteomics & Mass Spectrometry core
The ICBR Proteomics & Mass Spectrometry core now offers services using the newly acquired Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer. This instrument is the newest generation of the popular Orbitrap series. It generates high quality MS and MS/MS data and is capable of obtaining 140,000 resolution and <1 ppm mass accuracy. Its advanced quadrupole technology improves quantitation of low-abundance ions in complex matrices. In addition to high-throughput quantitation and identification, the QE+ provides new capabilities, including:
- Data-independent analysis (DIA) to acquire quantitative data with qualitative confirmation.
- Selected-ion monitoring (SIM) for targeted and sensitive quantitation in samples of medium complexity.
- Parallel-reaction monitoring (PRM) for highly specific and sensitive quantitation in samples of high complexity.
The instrument is coupled with a Thermo EASY-nLC1000 for LC-MS experiments. This acquisition benefits our current workflow and offers new capabilities and advanced technology to be applied to your research projects.
Contact the Proteomics & Mass Spectrometry core at ICBR-Proteomics@ad.ufl.edu for more information about all available services.