Serratia liquefaciens Genome Sequencing Accomplished in Two Weeks on PacBio SMRT System
The ICBR NextGen DNA Sequencing core laboratory sequenced and assembled a whole bacterial genome, Serratia liquefaciens, in a matter of days using the Pacific Biosciences (PacBio) Single Molecule, Real-Time Sequencing (SMRT) system.
Although ICBR has been involved with previous bacterial whole genome sequencing projects, the remarkably long continuous read lengths of the PacBio system enables whole microbial genome sequencing in considerably less time than previous methods. The extremely long PacBio reads allow for a near complete contiguous assembly of an entire 5-6 Mb bacterial genome in one shot.
The Serratia liquefaciens genome was announced August 15 in Genome Announcements:
Nicholson, W.L., Leonard, M.T., Fajardo-Cavazos, P., Panayotova, N., Farmerie, W.G., Triplett, E.W., & Schuerger, A.C. (2013). “Complete Genome Sequence of Serratia liquefaciens Strain ATCC 27592.” Genome Announcements, 1(4), e00548-13.
Andrew Schuerger, PhD, primary investigator and Plant Pathology Research Assistant Professor, located at the Space Life Science Lab at Kennedy Space Center, studies S. liquefaciens as part of his research on plant pathogens and the survival of terrestrial microorganisms under Martian atmospheric conditions. Schuerger previously demonstrated S. liquefaciens survives and grows in the extreme low temperature, low pressure, and anoxic conditions present on Mars.
Whole genome sequencing is the first step toward identifying the genetic makeup of microbes in the quest to understand their remarkable growth and survival under extreme environmental conditions.