Publication: Identifying predictive biomarkers of necrotizing enterocolitis in low birth weight infants

Published: October 11th, 2013

Category: All News, Announcements, Featured News, Proteomics and Mass Spectrometry

Necrotizing enterocolitis (NEC) is a serious disease that is most frequent among premature or sick infants, and is a major cause of death and morbidity in very low birth weight infants. The ICBR Protein Biomarker core laboratory, working with R.M. Torrazza and UF Health Pediatrics professor Josef Neu, used 2-D DIGE technology to explore the differences between protein contents of healthy babies and those affected by NEC to identify biomarker(s) from buccal swab samples that may predict NEC.  

Torrazza, R.M., Li, N., Young, C., Kobeissy, F., Chow, M., Chen, S., Mai, V., Sharma, R., Hudak, M., Shuster, J., Neu, J. (2013) Pilot study using proteomics to identify predictive biomarkers of necrotizing enterocolitis from buccal swabs in very low birth weight infants. Neonatology, 140(3), 234-242.

The figure below is the 2-D protein map overlay of the buccal swab protein extracted from healthy babies (Cy5 labeled, red), NEC babies (Cy3 labeled, green), and internal reference (Cy2 labeled, blue). From the differential protein abundance analysis, 37 altered proteins were found and three protein candidates were chosen for this study, Interleukin-1 receptor-antagonist (IL-1RA), but not peroxiredoxin and alpha-1 antitrypsin.

2-color image of buccal swab protein abundance analysis between normal and NEC premature baby. 100 ug each of Cy3-case 2 (J257 Wks 1-3), Cy5-control 2 (J255 wks 1-3) , and Cy2- internal reference were mixed and was subjected to IEF in a 24 cm pH 3 to 11 non linear IPG strip at 10k Volt until steady state before analyzed in a large 8 to 16% Tris Glycine SDS PAGE.

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