New protein phosphorylation site mapping workflow
Protein phosphorylation and dephosphorylation constitutes one of the reversible posttranslational mechanisms that control gene expression, signal transduction, cell cycle, apoptosis and cytoskeletal regulation. Due to its dynamic nature, stoichiometry of phosphorylation is usually low and presents a challenge for phosphoprotein analysis.
To overcome this challenge, ICBR Proteomics division has developed an effective workflow, which includes phosphopeptide enrichment and advanced mass spectrometry for phosphoproteomics and targeted phosphoprotein analysis. This workflow has been applied to several projects aimed at discovering novel phosphoproteins in signaling pathways and validating specific phosphorylating sites on interesting kinases and substrates.
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