Proteomics Highlights
Bioinformatics | Cellomics | Genomics | Proteomics
The following items represent some of the more significant research publications and findings that the Proteomics division has been a part of. For more information please contact Sophie Alvarez, PhD.
- Assessment of Acrylamide-induced Neurotoxicity Using Proteomics-based Technologies
- A recent proteomic study provided evidence that N-ethylmaleimide sensitive factor (NSF), a rate-limiting ATPase involved in presynaptic transmitter release, was a target of in vivo or in vitro ACR exposure [1]. To confirm and extend these initial findings, liquid chromatography-tandem mass spectrometry (LC/MS/MS) was used to identify ACR protein adducts in tryptic digests of synaptosomes isolated from moderately ACR-exposed rats. Analyses using isotope-coded affinity tags (ICAT) revealed quantitative changes in the relative abundance of many cysteine-containing peptides (indicating potential modification by ACR) derived from synaptosomal proteins. These findings will provide a better understanding of the mechanisms involved with presynaptic accumulation of ACR adducts and its effect on synaptic function.
- Barber, D. S.; LoPachin, R. M.; "Proteomic Analysis of Acrylamide-Protein Adduct Formation in Rat Brain Synaptosomes"; Toxicol. Appl. Pharmacol., 201, 120-136 (2004).
- Stevens Jr., S. M.; Barber, D. S.; "Mass Spectrometric Analysis of Synaptosomal Protein Modification Upon Exposure to Selected a, ß-Unsaturated Carbonyl Compounds"; 54th ASMS Conference on Mass Spectrometry, Seattle, WA (2006).
- Development of a Fluorescent Affinity Tag for Phosphoprotein Detection
- ICBR Proteomics has developed a novel chemical compound [1,2] to improve detection of phosphorylated proteins. For further information visit the University of Florida Office of Technology Licensing website (http://apps.rgp.ufl.edu/otl/viewTechInfo.cfm?case=11602).
- Stevens Jr., S. M.; Chung, A. Y.; Chow, M. C.; McClung, S. H.; Strachan, C. N.; Harmon, A. C.; Denslow, N. D.; Prokai, L.; "Enhancement of Phosphoprotein Analysis Using a Fluorescent Affinity Tag and Mass Spectrometry"; Rapid Commun. Mass Spectrom., 19, 2157-2162 (2005).
- Stevens Jr., S. M.; Chung, A.; Chow, M.; Kirkland, P. A.; Gil, M. A.; Maupin-Furlow, J. A.; "Advancements in Posttranslational Modification Analysis Using a Fluorescent Affinity Tag and Mass Spectrometry"; ABRF conference, Long Beach, CA (2006).