Publication: Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

Published: December 12th, 2012

Category: All News, Announcements, Bioinformatics, Latest Publications, Sanger Sequencing

Bill Farmerie, Fahong Yu, Savita Shanker, and JiQiang Yao, scientists at the UF ICBR, have contributed to a recent, highly accessed publication in the journal BMC Developmental Biology:

Ozawa et al.(2012) Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. BMC Developmental Biology, 12(33).

Peter J. Hansen, UF Department of Animal Sciences and D.H. Barron Reproductive and Perinatal Research Program, led the study that determined, for the first time, global gene expression patterns in the inner cell mass (ICM) and trophectoderm (TE) isolated from bovine blastocysts.

A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM and TE in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.

Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.

This research was supported by Agriculture and Food Research Initiative Grant Nos. 2009-65203-05732 and 2011-67015-30688 from United States Dept. of Agriculture National Institute of Food and Agriculture and by a grant from the Research Opportunity Fund, University of Florida Research Foundation.

View other biotechnology research publications from ICBR scientists

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