ICBR: Service Fees

» Billing Information

Search Service Fees by Division



Bioinformatics
Cellomics:
Genomics:
Proteomics:

Back to Top

Bioinformatics Service Fees


Bioinformatics » Services | Service Fees | Instrumentation | Staff | How Do I? | Links

Service
UF NP COM
Bioinformatics Consulting – Microarray Analysis
$50 $75 $100
Bioinformatics Consulting – Sequence Analysis
$50 $75 $100
Bioinformatics Consulting – System Administration
$50 $75 $100
Bioinformatics Laptop Rental (per month)
$500 $750 $1,000
BLAST Search (per 1000 sequences)*
$50 $75 $100
BlastQuest (per project, per year)
$500 $750 $1,000
Custom Microarray Design (<50K input sequences)
$3,000 $4,500 $6,000
Custom Microarray Design (>50K input sequences)
$5,000 $7,500 $10,000
EST Assembly (per 1000 sequences)*
$50 $75 $100
FGENESB (per genome)
$500 $750 $1,000
MASCOT Search (per project)
$500 $750 $1,000
Microarray Analysis (~20 arrays)
$1,000 $1,500 $2,000
Microarray Analysis (~50 arrays)
$2,000 $3,000 $4,000
Microarray Analysis (100 – 200 arrays)
$5,000 $7,500 $10,000
myCAP private access (per project, per year)
$500 $750 $1,000
myCAP public access (per project, per year)
$300 $450 $600
UF-GENOME.ORG (per design)
$500 $750 $1,000
UF-GENOME.ORG (per project, per year)
$200 $300 $400

*There is a $100 setup fee for this service.
Back to Top | Download (.pdf, size: 6KB) (.xls, size: 19KB) — Prices subject to change

Cellomics Service Fees


Cellomics » Services | Service Fees | Instrumentation | Staff | How Do I? | Links

Electron Microscopy & Bioimaging (Byung-Ho Kang)
Service
UF NP COM
Hourly use of TEM
$35
$45
$60
Hourly use of TEM/assisted
$60
$90
$120
Hourly use of SEM
$35
$45
$60
Hourly use of SEM/assisted
$60
$90
$120
Hourly use of Zeiss confocal microscope
$35
$45
$60
Hourly use of Zeiss confocal microscope/assisted
$60
$90
$120
Olympus Flourescent/Brightfield microscope with image Q camera
$15
$25
$55
Standard TEM thin section prep per sample
$150
$290
$460
Additional similar samples submitted at the same time up to four
$120
$210
$350
Fixation, embedding, and polymerization
$65
$95
$185
Sectioning and staining 1 block and 3 grids
$65
$95
$185
Observe and photograph per hour
$60
$90
$120
TEM thin section prep and immunogold labeling or other cytochemical reaction, per sample (one antibody supplied by the researcher)
$175
$315
$550
Immunogold labeling only per antibody
$40
$75
$105
Negative staining or other whole mount preparation for TEM per sample
$45
$90
$140
SEM sample – long prep with critical point drying per sample
$120
$210
$330
Additional similar samples submitted at the same time up to four
$90
$160
$270
SEM sample – long prep with HMDS (hexamethyldisilazane) per sample
$90
$170
$280
Additional similar samples submitted at the same time up to four
$70
$130
$215
SEM sample – short prep per sample
$65
$110
$190
Microwave oven (for faster infiltration) per 0.5 hr minimum charge – 0.5 hr
$5
$8
$10
Ultramicrotome (supernova) supplies not included
$5
$8
$10
Laser jet prints per page
Free
$0.20
$0.30
Prints (high resolution) on photo paper
$1
$2
$3
Cryostat HM505E with cryo-Jane tape system per hour, minimum charge – 1 hr
$15
$25
$55
Sputter coater
$15 $25 $55
Critical point dryer
$15 $25 $55
Rotary shadow/Carbon evaporation per sample
$15 $25 $55
EM kits, supplies, and reagents per unit
$1 $2 $3
Assistance per hour
$30 $70 $130

Back to Top | Download (.pdf, size: 8KB) (.xls, size: 20KB) — Prices subject to change

Flow Cytometry (Neal Benson,MS)
Service
UF NP COM
Sample analysis, by staff
$55 $82.50 $110
Sample analysis, self-service
$33 Inquire Inquire
Cell sorting (by staff only)
$60 $90 $120
Off-line data analysis and manipulation, by staff
$25 $37.50 $50
Off-line data analysis, self-service
Free Inquire Inquire
Confocal 1st Hour/UF Self-User
$60 N/A N/A
Confocal Subsequent Hours/UF Self-User
$40 N/A N/A
Confocal 1st Hour w/ Assistance & Training
$75 $112.50 $150
Confocal Subsequent Hours w/ Assistance & Training
$55 $82.50 $110

*A 2-hour minimum charge will be applied to cell sorting projects.
Back to Top | Download (.pdf, size: 5KB) (.xls, size: 19KB) — Prices subject to change

Hybridoma (Linda Green)
Service
UF NP COM
IMMUNIZATION: Immunize mice in preparation for fusion, includes first six months per diem (each mouse)
$140 $170 $195
CELL FUSION: fuse cells, provide hybridoma supernatants for screening
$700 $1000 $1200
ELISA SCREENING: 96 well plate format (each plate)
$35 $70 $75
WESTERN SCREENING: Minigel format (each blot)
$90 $150 $180
CLONING: single cell cloning, cryopreservation of two monoclonal cell lines. Includes isotyping and mycoplasma testing of 1º clone
$400 $550 $650
IN VITRO ANTIBODY PRODUCTION: cell culture supernatant. Many different volumes available from 25 mls to 1000 mls—please inquire
Inquire Inquire Inquire
IN VITRO HIGH DENSITY ANTIBODY PRODUCTION Integra CelLine flask CL-350 RUN: 3 harvests
$350 $450 $550
PURIFICATION: standard scale using 5.0 ml protein G cartridge
$475 $600 $700
DEPOSIT: Thaw,grow, cryopreserve hybridoma or non-hybridoma cell line
$120 $150 $175
ISOTYPING: Determine subclass of mouse monoclonal antibodies
$30 $36 $42
MYCOPLASMA TESTING
$70 $100 $130
CRYOSTORAGE CHARGE : to be paid yearly for storage of uncloned hybridoma cell lines (beyond a 2 year grace period) ( each cell line)
$20 $40 $50

Back to Top | Download (.pdf, size: 7KB) (.xls, size: 18KB) — Prices subject to change

Genomics Service Fees


Genomics » Services | Service Fees | Instrumentation | Staff | How Do I? | Links

Realtime PCR (Li Zhang, PhD)
Service
UF NP COM
Instrumentation use for end point post PCR read (SNP assay)
$60 $90 $120
Absolute quantification data analysis
$30 $45 $60
Instrumentation use for Taqman Real Time PCR (384 wells or low density array, per run)
$50 $75 $100
Instrumentation use for Syber Green Real Time PCR (96 wells plate, per run)
$40 $60 $80
Instrumentation use for Taqman Real Time PCR (96 wells plate, per run)
$40 $60 $80
Primer-Probe design (per gene)
$30 $45 $60
Primer-Probe sets per 20 reaction
$60 $90 $120
Consultation and data interpretation (per hour)
$50 $75 $100
Taqman qPCR (per sample/per gene)
$20 $30 $40
Taqman RT-qPCR (per sample/per gene)
$30 $45 $60
Syber Green qPCR (per sample/per gene)
$15 $22 $30
Syber Green RT-qPCR (per sample/per gene)
$25 $38 $50
Taqman chemistry qPCR optimization
$950 $1,425 $1,900
Syber Green chemistry qPCR optimization
$1,250 $1,875 $2,500
Alternative splice forms
$36 $54 $72
Result printed report (per page)
$0.60 $0.80 $1.20
RNA clean-up (per sample)
$22 $33 $44
Taqman qPCR (per reaction)
$7 $10.50 $14
Syber Green qPCR (per reaction)
$5 $7.50 $10
100 qPCR reaction kit
$150 $225 $300
Software copy
$250 $375 $500
Hands on tanning (per day)
$400 $600 $800
Relative quantification study (96wells plate)
$40 $60 $80
qPCR product quality analysis (up to 12 samples)
$100 $150 $200
qPCR product quantity analysis (per sample)
$10 $15 $20
Relative quantification study (384 wells plate or low density array)
$120 $160 $240
Syber Green qPCR dissociation carve
$10 $15 $20
Reverse Transcription (per sample)
$50 $75 $100
Plate/Optic-cap (per plate)
$10 $15 $20
Instrumentation use for TaqMan Real Time PCR (more then 5 plates/month)
$40 $60 $80
Instrumentation use for SYBR Green Real Time PCR (more then 5 plates/month
$40 $60 $80
TaqMan primer & probe/Gene
$285 $285 $285

Back to Top | Download (.pdf, size: 8KB) (.xls, size: 21KB) — Prices subject to change

Sanger Sequencing (Savita Shanker, PhD)
Service
UF NP COM
Reaction, Un-edited
$8 $9 $11
Reaction, 600 bases, Edited
$10 $11 $13
Reaction,800 –1100 bases, Edited
$12 $13 $15
Miscellaneous
$1 $2 $3
Reaction,Re-sequenced but failed
$8 $9 $11
Load only, unedited
$3 $4 Inquire
Load only, failed run
$3 $4 Inquire
Load only, up to 600 bases, Edited
$5 $6 Inquire
Load only, 700-1100 bases edited
$7 $8 Inquire
96reactions/purified DNA provided in tubes, Re-sequenced,Un-edited (/Reaction)
$6 $6 Inquire
96reactions/provide purified DNA in tubes, Re-sequenced,Edited(/Reaction)
$7.50 $7.50 Inquire
Chromatogram Color printout
$2 $2 Inquire
Primer Design (per primer)
$5 $5 $5
Sequence Alignments, editing, assembly/KB
$5 $5 Inquire
Big dye Mix(/8ul)
$7.50 $7.50 Inquire
Signal enhancer/reaction
$0.25 $0.25 Inquire
Primer synthesis
$8 $8 Inquire
96Well format only, provide Bacterial cultures, sequencing, no re-runs, Un-edited (/plate)
$288 $288 $288
96well format only, provide colonies, sequencing, no re-runs, Un-edited (/plate)
$ 312 $312 $312
96 well format only, PCR/exo-sap, sequencing, no re-runs,Un-edited (/plate)
$264 $264 $264
96 well format, provide purified PCR product, sequencing, no re-runs, Un-edited (/plate)
$249 $249 $249
Primer surcharge/plate/primer for >2 primers
$50 $50 $50
Large projects
Inquire Inquire Inquire
Colony picking-source plate charge
$2 $2.86 $2.86
Bacterial Library Plate per Q-Tray for auto colony
$30 $42.90 $42.90
Automated Colony Picking-96 well destination plate
$6 $8.58 $8.58
Automated Colony picking-384 well destination plate
$10 $14.30 $14.30
Colony Gridding-source plate charge
$2 $2.86 $2.86
Macroarray Gridding
$50 $71.50 $71.50
Library plate Replication-per destination plate
$6 $8.58 $8.58
Library Plate Replication-per source plate
$2 $3 $3
Library Re-arraying-per 96 well destination plate
$10 $14.30 $14.30
Library Re-arraying-per 384 well destination plate
$20 $28.60 $28.60
Library Re-arraying per source plate
$3 $4.29 $4.29
Macro-array Gridding-duplicate membrane
$10 $14.30 $14.30
Kits, Supplies, Reagents
$1 $2 $3
Consultation
$10 $20 $30

Back to Top | Download (.pdf, size: 9KB) (.xls, size: 24KB) — Prices subject to change

454 Sequencing (Regina Shaw)
Service
UF NP COM
454 Library Construction and Titration on FLX 16-region 70x75 PTP, 1 library
$2,760 $3,180 Inquire
454 Library Construction and Titration on FLX 16-region 70x75 PTP, 2 libraries
$5,050 $5,800 Inquire
454 Library Construction and Titration on FLX 16-region 70x75 PTP, 3 libraries
$7,530 $8,660 Inquire
454 Library Construction and Titration on FLX 16-region 70x75 PTP, 4 libraries
$10,000 $11,500 Inquire
454 Production run on FLX 70x75 PTP, full plate
$10,800 $12,400 Inquire
454 Sample Preparation for Titration on 70x75 PTP (No Library Construction)
$80 $90 Inquire
454 Library Construction, 1 library (No Titration Sequencing Run)
$570 $660 Inquire
454 Paired End Library Construction, 1 library
$220 $250 Inquire
454 Sample Preparation and Testing for Amplicon Sequencing (No Sequencing Run)
$195 $225 Inquire

Back to Top | Download (.pdf, size: 6KB) (.xls, size: 18KB) — Prices subject to change

Genetic Analysis (Ginger Clark, MS)
Service
UF NP COM
Genomic DNA Isolation
$8 $10 $12
PCR Reaction
$8 $10 $12
PCR Reaction Purification
$8 $10 $12
Mitochondrial DNA Analysis (Inc Isol, PCR, Purif)
$60 $120 $180
T4 DNA Ligase Reaction
$10.50 $21 $31.50
T-Vector Cloning
$35 $70 $105
Plasmid Preparation (small scale)
$12 $24 $36
Restriction Enzyme Digestion
$7.50 $15 $22.50
Oligonuclotide Primer/Probe Design
$25 $50 $75
Microsatellite Analysis
$12 $18 $18
Fragment Analysis (MegaBACE or ABI 3730)/ lane
$1 $1 $1
Visiting Scientist Lab Fees (per week)
$250 $250 $250
Enriched Microsatellite Library Development
Microsat. Library Develop. & Screen
$8,000 $10,000 $12,000
Dot Blot/Colony Screen
$75 $150 $225
T-Vector cloning
$35 $70 $105
Plasmid Preparation (small scale)
$12 $24 $36
Restriction Enzyme Digestion
$7.50 $15 $22.50
Oligonuclotide Primer/Probe Design
$25 $50 $75
Primer Optimization (per primer)
$150 $300 $450
Microsatellite Polymorphism Screen (per primer)
$150 $300 $450
Forensic Fees (Wildlife and Human)
Species Determination (per sample)
$150 $150 $300
Gender Determination (per sample)
$100 $100 $200
DNA Fingerprinting (Genetic Profile) per sample
$200 $200 $400
Species and Gender (per sample)
$187.50 $187.50 $375
Species, Gender, and Genetic Profile (per sample)
$325 $325 $650
Human STR Typing (8 loci) per sample
$250 $250 $500
Human mtDNA Typing (HRVI and II) per sample
$250 $250 $500
Tissue Sample vials (20 minimum) per vial
$0.50 $0.50 $0.50

Back to Top | Download (.pdf, size: 8KB) (.xls, size: 20KB) — Prices subject to change

SNP Genotyping (David Moraga, PhD)
Service
UF NP COM
GoldenGate Assay Custom Assays and Arrays
96 SNPs panel
$7,043.40 Inquire Inquire
192 SNPs panel
$9,289.80 Inquire Inquire
384 SNPs panel
$13,033.80 Inquire Inquire
512 SNPs panel
$17,199 Inquire Inquire
768 SNPs panel
$22,440.60 Inquire Inquire
1536 SNPs panel
$40,480.70 Inquire Inquire
GoldenGate Assay Standard Assays and Arrays
MHC Panel Set 2360 SNPs
$16,276 Inquire Inquire
MHC Mapping Panel 1293 SNPs
$8,489 Inquire Inquire
MHC Exon-Centric Panel  1228 SNPs
$8,450 Inquire Inquire
Cancer SNP Panel 1421 loci, 400 genes
$8,593 Inquire Inquire
HumanLinkage V Panel, 6K loci
$31,902 Inquire Inquire
Mouse LD Linkage 377 loci
$5,083 Inquire Inquire
Mouse MD Linkage 1449 loci
$8,619 Inquire Inquire
Whole Genome Genotyping
Infinium HumanNS-12 Genotyping Kit  11k Nonsynonymous SNPs, 8 Beadchips
$12,355.20 Inquire Inquire
Infinium HumanHap240S Whole-Genome Genotyping Kit, 8 Beadchips
$4,680 Inquire Inquire
HumanHap550 v3.0 Whole-Genome Genotyping Kit, 8 Beadchips
$7,280 Inquire Inquire
HumanHap650Y v3.0 Whole-Genome Genotyping Kit, 8 Beadchips
$8,008 Inquire Inquire
HumanCNV370-Duo v1.0 DNA Analysis Kit, 8 Beadchips
$3,640 Inquire Inquire
Whole Genome Expression (Microarrays)
Sentrix Human-6_v2 Expression BeadChip, 6-pack, 47K genes
$7,488 Inquire Inquire
Sentrix HumanRef-8_v2 Expression BeadChip, 6-pack, 23K genes
$6,240 Inquire Inquire
Sentrix Mouse-6 v1.1 Expression BeadChip (6), 47K genes
$7,488 Inquire Inquire
Sentrix MouseRef-8 v1.1 Expression BeadChip (6), 24K genes
$6,240 Inquire Inquire
Sentrix RatRef-12 Expression BeadChip, 6-pack, 24K genes
$9,360 Inquire Inquire
Gene Expression Focused Products (Microarrays)
Sentrix STD Human Sampler-16 Expression BeadChip (1), 516 genes each
$1,248 Inquire Inquire
Sentrix Custom-16 Expression BeadChip (6 pack), 14,700 genes
$7,488 Inquire Inquire
Special Services
Special Service
Inquire Inquire Inquire

Minimum amount of sample/single assay to be submitted per type of assay should be as followed: GoldenGate (500 ng @ 50 ng/ul), Infinium (1 microgram @ 50 ng/ul), Whole Genome Expression (100 ng @ 10 ng/ul), DNA Methylation (1 microgram @ 50 ng/ul).
Back to Top | Download (.pdf, size: 9KB) (.xls, size: 21KB) — Prices subject to change

Gene Expression (Gigi Ostrow, PhD)
Service
UF NP COM
Agilent
Bioanalyzer Run (1-12 samples) (per run)
$35 $40.25 $43.75
Complete Target Labeling for Microarray (per sample)
$350 $402.50 $437.50
Hybridization and Scan Only (per slide)
$85 $97.75 $106.25
Scan Only (per scan)
$40 $46 $50
Affymetrix
Complete GeneChip Chemistry for Microarray (sample >5ug total RNA) (per sample)
$500 $575 $625
Hybridization and Scan Only (per slide)
$60 $69 $75
Please be aware that, for microarray experiments, clients are requested to purchase their chips and bring those to the core along with their samples.
Do not forget the gasket slides when ordering Agilent chips.

Back to Top | Download (.pdf, size: 6KB) (.xls, size: 20KB) — Prices subject to change

Proteomics Service Fees


Proteomics » Services | Service Fees | Instrumentation | Staff | How Do I? | Links

Mass Spectrometry and Protein Characterization (Scott McClung)
LC-MS/MS Requisition Form (.pdf), MALDI-TOF/TOF Requisition Form (.pdf)
Service
UF NP COM
Conjugations - Maleimide to KHL/Ovalb
$180 $200 $400
Edman - Protein Seq Setup
$110 $120 $200
Edman - Protein Seq Cycles, per amino acid
$18 $20 $38
Edman - Protein Seq Setup (PVDF - 4 and higher samples)
$50 $60 $100
AAA - Hydrolysis, per sample
$25 $35 $75
AAA - Analysis, per sample
$40 $55 $120
HPLC - Collection of fractions, per sample
$50 $100 $150
Trypsin digestion, per sample (Manual)
$10 $15 $20
ZipTip, per sample (Manual)
$5 $8 $10
MALDI-TOF/TOF, PMF (Peptides), per sample
$30 $35 $50
MALDI-TOF/TOF, PMF (Protein), per sample
$30 $35 $50
MALDI-TOF/TOF, self service, per hour
$15 $50 $100
MALDI-TOF/TOF automatic workflow (digestion included), set up fee 
$75 $105 $135
MALDI-TOF/TOF automatic workflow (digestion included), PMF, per sample ID only if >20 (+ set up fee) 
$10 $15 $30
MALDI-TOF/TOF automatic workflow (digestion included), PMF and MS/MS, per sample ID only if >20 (+ set up fee) 
$15 $25 $50
QSTAR - direct infusion, per sample
$30 $60 $90
LC-MS/MS - Gel spot or band - 20min gradient for protein ID, per sample 
$50 $75 $150
LC-MS/MS - Gel spot or band - 20min gradient for protein ID, per sample if > 10
$35 $45 $110
LC-MS/MS - Gel spot or band - 20min gradient for protein ID, per sample if in-house
$30 $40 $100
LC-MS/MS - In solution sample - 1h gradient for protein ID, per sample 
$80 $115 $250
LC-MS/MS - In solution sample - 1h gradient for protein ID, per sample if > 10
$45 $70 $190
LC-MS/MS - In solution sample - 2h gradient for protein ID of complex mixture, per sample
$140 $200 $350
LC-MS/MS - In solution sample - 2h gradient for protein ID of complex mixture, per sample if > 5
$85 $160 $280
LC-MS/MS - In solution sample - 2h gradient for protein ID of complex mixture, per sample if > 10
$60 $120 $240
Quantitative Proteomic - iTRAQ samples labelling, 2 to 4 plex (not including LC-MS/MS runs)
$350 $450 $750
Quantitative Proteomic - Label free, set up fee for 1 experiment (2 to 4 samples)
$90 $120 $160
Method development for special projects, per hour
$40 $80 $160
(HPLC fractionation; Post-translational modifications; Quantitative Small Molecules/Peptides/Phosphopeptides)

Back to Top | Download (.pdf, size: 9KB) (.xls, size: 21KB) — Prices subject to change

Peptide Synthesis (Alfred Chung, MS)
Peptide Synthesis Requisition Form (.pdf)
Service
UF NP COM
Pep. Synth - set-up 25 uMole (includes 5AA)
$100 $125 $250
Pep. Synth - set-up 100 uMole (includes 5AA)
$200 $225 $400
Pep. Synth - 100 uM, per amino acid
$40 $45 $80
Pep. Synth - 25 uM, per amino acid
$20 $25 $50
Pep. Synth - 250 uM, per amino acid
$55 $60 $100
Peptide Purification, 1-5 mg
$50 $60 $100
Peptide Purification, 10-25 mg
$200 $220 $400
Peptide purification, 6-10 mg
$100 $110 $200
Pep. Synth-Crude (batches of 10)-per amino acid
$12 $15 $15

Back to Top | Download (.pdf, size: 5KB) (.xls, size: 17KB) — Prices subject to change

2-D Difference Gel Electrophoresis (Marjorie Chow, MS)
2D Gel Electrophoresis Requisition Form (.pdf)
Service
UF NP COM
mini 1D PAGE, 7x8 cm
$50 $75 $100
extra large, 2D 22x22 cm
$150 $225 $300
BIAcore Analysis, self service, per day charge
$80 $120 $160
BIAcore Analysis, contract by staff, per day charge
$200 $300 $400
one day training on using BIACORE
$400 $600 $800
BIAcore Start-up kit
$170 $255 $340
CyDye, minimum charge per 100 ug protein per dye
$160 $240 $320
CyDye, extra CyDye (50 ug protein/dye)
$80 $120 $160
Colloidal blue stain per 50 ml
$5 $7.50 $10
SilverQuest stain per 50 ml
$10 $15 $20
Sypro Ruby stain per 50 ml
$15 $22.50 $30

Back to Top | Download (.pdf, size: 6KB) (.xls, size: 18KB) — Prices subject to change

Service Fee Groups



The ICBR has three Service Fee groups:

UF

University of Florida

NP

Non-Profit entities including all non-UF colleges and universities, state and federal agencies, and non-profit hospitals and research institutions.

COM

Commercial for-profit entities

Billing Information



University of Florida Customers

UF customers may pay for ICBR Services through PeopleSoft (PS) either Unencumbered or with a UF Purchase Order (PO). To create a UF Purchase Order through PeopleSoft, click here for step-by-step instructions.

When you receive your ICBR Invoice, please write the correct PS Chartfield or the PO Number on the Invoice. Please Fax (352-273-8070) or Mail the Invoice to ICBR, PO Box 103622.

All Other Customers

ICBR will mail the Invoice for payment when the work is completed. If you wish to submit a Purchase Order, please Fax (352-273-8070) or Mail the PO to:

ICBR
University of Florida
PO Box 103622
Gainesville, FL 32610
ATT: Sandy Gibbons

When you submit samples or an ICBR service request, please reference your internal Purchase Order Number with each order so that we can Invoice the service(s) against the correct PO.

Billing Questions Contact

Sandy Gibbons

Back to Top