ICBR is expert and experienced in helping researchers prepare proposals by providing letters of support, budget information, project method descriptions and other information for use in proposal submission. ICBR staff can also be engaged as Co-Investigators, Key Personnel or other support staff, thereby allowing their CV with demonstrated expertise to be included in proposal submissions.The inclusion of ICBR staff and services in a proposal firmly establishes that work will be completed with professional expertise, both broadening and strengthening proposals.
A summary of particular core services can be found below. Use of these descriptions with proposals is welcome and encouraged. Potential ICBR customers are strongly encouraged to work directly with ICBR staff to get additional specific details on budget items, staff expertise, and equipment descriptions.
The Bioinformatics core offers bioinformatics and biostatistics consulting and data analysis services to help researchers toward an in-depth understanding of large-scale data sets…more.
The Cytometry core provides a variety of tools and expertise for cellular measurements. The laboratory incorporates numerous flow cytometers, from simple entry-level devices…more.
Electron Microscopy Core
The Electron Microscopy core provides microscopic imaging services on a fee-for-service basis. The core is equipped with a set of microscopes having imaging capability from…more.
Gene Expression & Genotyping Core
The Gene Expression & Genotyping core provides a variety of gene expression services related to Affymetrix and Agilent array process including whole genomic wide gene expression arrays, miRNA array, DNA…more.
The core also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. We also set up most types of fragments for analysis on the AB3730xl automated sequencer; providing the buffers and size standards. The AdvanCE FS96 is a 96 capillary instrument used to analyze fragments with greater than trinucleotide repeats. In addition, the Genotyping core designs, characterizes, and processes microsatellite loci used for individual identification, population studies, and geneflow. The core has an Illumina BeadArray Reader used to analyze SNP and gene expression samples in a high-throughput fashion.
We also provide real-time PCR services by utilizing Applied Biosystems (ABI) 7500 FAST, ABI 7900HT, and BioRad CFX96. We support both SybrGreen and TaqMan reaction chemistries. Our 7900HT system is equipped with both 96- and 384-well blocks to facilitate high-throughput analysis. Applications include quantitative gene expression analysis, pathogen detection/quantification, and allelic discrimination (SNP detection). Our walk-up self service on the AB 7500 FAST allows researchers to set-up and run their own plates, but not purchase their own machine. The QUANTSTUDIO 3d DPCR SYSTEM allows customer to do absolute quantification, rare allele detection, quantification using TaqMan SNP array, and CNV (Copy Number Variation) in a more sensitive, specific, and precise way.
Monoclonal Antibody Core
The primary service of the Monoclonal Antibody core is the development of new mouse monoclonal antibodies. All phases of antibody development are carried out by this laboratory…more.
NextGen DNA Sequencing Core
The NextGen DNA Sequencing core at the Interdisciplinary Center for Biotechnology Research (ICBR) provides researchers with quality, massively parallel, high-throughput sequencing data using the most current instrumentation, at a reasonable cost. We currently support the most popular platforms: Illumina NextSeq500 and MiSeq, HiSeq3000 and the Pacific Biosciences SEQUEL system. Together, these instruments cover a broad range of powerful applications, thus allowing biologists to investigate and obtain answers to questions that were not attainable until recently. Our free consultation services aim to help researchers navigate through the complex matrix of experimental options represented by the available sequencing technologies. Some of these important considerations include: read length, error rate, predominant type of error, data output/run, speed, cost, etc. The ever-broadening range of applications include (but are not limited to) de novo sequencing of whole genomes, targeted sequencing, transcriptome sequencing (RNA-seq), chromatin immunoprecipitation (ChIP) sequencing, methylation analysis, and metagenomics.
Proteomics & Mass Spectrometry Core
The Proteomics & Mass Spectrometry core provides a variety of services related to protein fractionation, qualitative and quantitative mass spectrometry analysis, de novo protein sequencing and…more.
The core also provides a variety of services related to Biomarker Discovery, such as protein abundance analysis using gel-based 2 -D Fluorescence Difference Gel Electrophoresis (2-D DIGE); protein separation using 1-D or regular 2-D gel electrophoresis and Western blotting; 2-D gel-based phosphor protein and glycoprotein profiling. For these services we have instruments such as Ettan IPGphor, Ettan DaltSix, Typhoon Trio+ phosphor scanner, Investigator ProPic automatic spot picker. In addition, we have image analysis softwares such as GE’s DeCyder and Non-Linear Dynamics SameSpot Progenesis with Stats. Furthermore, the core provides protein enrichment and abundant protein depletion from biological fluids such as plasma, CSF, and urine using different techniques such as combinatory peptide (ligand) library, and assorted immunodepletion. We also provide liquid phase preparative IEF to fractionate protein or peptide mixture using an Agilent 3100 Offgel fractionator. Moreover, the core provides training on using Surface Plasmon Resonance (SPR) technology to study molecular interaction. We have Biacore 3000 for therapeutic drug discovery, antibody screening, and ligand fishing, and other applications including affinity measurements, binding kinetics, binding specificity, epitope mapping, and active concentration assay.