Single-Cell Genomic Analysis – C1™ Single-Cell Auto Prep System

Published: March 17th, 2015

Category: All News, Announcements, Featured News, Gene Expression and Genotyping

The Fluidigm C1™ Single-Cell Auto Prep system, now available through the ICBR Gene Expression & Genotyping core, allows researchers and core customers to rapidly and reliably isolate, process, and profile single cells for genomics analysis. It enables researchers to study cell differentiation, measuring single cell response to specific stimuli, verify critical disease biomarkers, validate RNAi knockdown, and conduct candidate drug screens. The C1 is easy to use with a streamlined workflow and intuitive interface as well as fast, offering cell input-to-data in less than a day. Through its plug-and-play workflow, 96 single cells can be reproducibly prepared in about one hour of hands-on time. Currently, there are four applications available through the ICBR Gene Expression & Genotyping core with the C1 Single-Cell Auto Prep System:

  1. Using C1 to generate single-cell cDNA libraries for mRNA sequencing: the protocols allow the user to capture cells, convert poly (A) RNA into full-length cDNA, and perform universal amplification of the cDNA using the Fluidigm C1 system and C1 integrated fluidic circuits (IFC). 96 individual cDNA libraries will then be completed with the Agilent Bravo NextGen Library Automation System.
  2. Using C1 to generate single-cell libraries for DNA sequencing: cells are lysed and the DNA denatured. Genome amplification is then performed with the C1 system and IFC. After whole genome amplification, targeted, whole genome sequencing or whole exome sequencing can be completed accordingly using the Agilent Bravo NextGen Library Automation System.
  3. Using C1 to capture cells from cell culture and perform pre-amplification of micro RNA species using TaqMan Assays.
  4. Using C1 to capture cells from cell culture and perform pre-amplification through TaqMan Assays.

For more information, please contact the Gene Expression & Genotyping core at ICBR-GeneExpression@ad.ufl.edu.

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